纳奥德生调节血管功能通过G蛋白结合受体:网络分析和实验调查
Shuhan Chen1, Ziran Niu1,2, Yanjia Shen1
1Beijing Key Laboratory of Drug Targets Identification and Drug Screening, Institute of Materia Medica, Chinese Academy of Medical Sciences and Peking Union Medical College, Beijing, China.
Frontiers in pharmacology
|March 27, 2024
概括
纳奥德生 (NDS) 是一种传统的中国草药,通过利用多种代谢物向G蛋白结合受体 (GPCRs) 来改善血液循环和血管度. 这项研究确定了关键化合物及其协同作用,提供了对其治疗大脑血管疾病的治疗潜力的见解.
科学领域:
- 药理学和中国传统医学
- 心血管研究研究心血管研究
- 神经科学是一个神经科学.
背景情况:
- 缺血性中风 (IS) 提供了有限的治疗选择,再通道化对恢复至关重要.
- 纳奥德申 (NDS) 是一种草药,在临床上用于脑血管疾病,但其机制尚不清楚.
- 了解NDS改善血液循环和血管度的物质基础是必不可少的.
研究的目的:
- 阐明Naodesheng (NDS) 在改善血液循环和调节血管度方面的物质基础.
- 为了在NDS中确定负责其脑血管影响的特定代谢物.
- 探索NDS的多组件和多目标机制.
主要方法:
- 为NDS (777种化合物) 构建化学代谢物数据库.
- 分子对接,以评估NDS代谢产物与五种血管调G蛋白结合受体 (GPCR) 之间的相互作用.
- 网络分析和实验室内血管运动实验,对大鼠的基本动脉环.
主要成果:
- NDS组件诱导了预先收缩的老鼠基础动脉环的剂量依赖放松.
- 来自C.的代谢产物 丁克托里乌斯,C. 皮纳蒂菲达,以及P. 诺托金生显示了高的对接得分.
- 特定的代谢物 (例如,异姆尼丁,凯姆菲罗尔,戴兹) 通过GPCR相互作用表现出显著的动脉放松 (>90%) 和协同效应.
结论:
- 通过多组件,多目标机制,NDS表现出血管调节效应.
- 具有中等至高结合能量的已识别的代谢物在NDS的血管功能中起着至关重要的作用.
- 这些发现为NDS在脑血管疾病中的使用提供了科学依据.
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