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鉴定与两种不同重组杂交的豆系种群中的种子蛋白度相关的QTLs

Krishna Kishore Gali¹, Ambuj Jha^1,2, Bunyamain Tar'an¹

¹Department of Plant Sciences, Crop Development Centre, University of Saskatchewan, Saskatoon, SK, Canada.

Frontiers in plant science

|March 27, 2024

概括

这项研究确定了提高豆种子蛋白度 (SPC) 的关键定量特征位置 (QTL). 这些发现支持标记辅助选择 (MAS) 开发高蛋白豆种类.

科学领域:

植物遗传学和育种.
农业科学农业科学
食品科学食品科学食品科学

背景情况:

对植物性蛋白质的需求不断增加,突出了提高豆 (Pisum sativum L.) 中种子蛋白质度 (SPC) 的需要.
标记器辅助选择 (MAS) 可以加速改良的豆品种的繁殖,具有更高的SPC.

研究的目的:

为了确定与SPC相关的定量特征位点 (QTL),在两个不同的豆复合同系 (RIL) 种群中.
支持标记器辅助选择 (MAS) 开发具有增强种子蛋白度的豆类品种.

主要方法:

使用SNP数组对166个 (PR-30) 和159个 (PR-31) RIL进行基因定型.
在加拿大萨斯喀彻温省的多个地点对农学特征和SPC进行表型化.
复合间隔映射用于识别与SPC和谷物产量相关的QTL.

主要成果:

在PR-30中确定了SPC的3个QTL,在PR-31中确定了5个QTL,LOD得分从3.0到11.0不等.
发现了三个显著的QTL (SPC-Ps-4.2,SPC-Ps-5.1,SPC-Ps-7.2) 解释了SPC的显著表型变异.
确定了8个谷物产量QTL,并观察了SPC和谷物产量QTL之间共享的区域.

结论:

确定了SPC的新型QTL,其中特定的QTL适用于标记器辅助育种,以增加豆类中的蛋白质含量.
对SPC的育种策略必须考虑对谷物产量的潜在影响,因为QTL地区可以影响这两种特征.
已识别的QTL为加速高蛋白豆品种的发展提供了宝贵的遗传资源.

关键词:

在 Pisum sativum 里面可以看到.在 QTL 时段,你会得到 QTL.通过SNP基因定型进行SNP基因定型用标记器辅助的选择.植物蛋白质是一种植物蛋白质.

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