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用设计的Aptamer显示粒子检测SARS-CoV-2的光度和色度测量方法.

Ki Sung Park1, Anna Choi1, Tae-In Park1

  • 1Department of Biotechnology and Bioinformatics, Korea University, Sejong 30019, Republic of Korea.

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概括

研究人员开发了新型的体显示粒子 (AdPs),用于快速,低成本的SARS-CoV-2检测. 这些ADP允许使用简单的度和色度测试进行敏感的诊断,为现有方法提供了一个有希望的替代方案.

关键词:
在SARS-CoV-2的阿帕特马体中.在SARS-CoV-2的尖端蛋白质结合型阿巴特马体中.在aptamer显示器中显示粒子.颜色测量应用传感器度吸食传感器是一种度吸食传感器.微粒显示器显示的粒子显示器.

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科学领域:

  • 生物技术是生物技术.
  • 纳米技术 纳米技术
  • 分子诊断学 分子诊断学

背景情况:

  • 由于COVID-19的流行,需要敏感和选择性的诊断工具.
  • 目前的诊断通常依赖于昂贵的抗体或酶和高科技设备.
  • 迫切需要快速,低成本的SARS-CoV-2检测方法.

研究的目的:

  • 开发和验证用于检测SARS-CoV-2的阿普塔默显示粒子 (AdPs).
  • 为SARS-CoV-2创建一个简单,低成本的诊断矩阵.
  • 评估基于AdP的测试的敏感性和选择性.

主要方法:

  • 产生两种不同的ADP (C1-AdP和C4-AdP),表现出特定的体.
  • 使用粒子PCR制造SARS-CoV-2检测矩阵.
  • 使用度和色度测试进行诊断.
  • 设计一种三明治类型的测试,使用针对SARS-CoV-2伪病毒的AdPs和aptamers.

主要成果:

  • AdPs对SARS-CoV-2尖端蛋白具有很高的结合亲和力,具有度依赖的光.
  • 在低度 (0.5 nM) 中检测阿巴胺结合.
  • 度测定实现了3.9 × 10^3伪病毒/mL的检测极限.
  • 放大色度测定达到广泛范围内的1 × 10 ^ 1伪病毒/mL的检测极限.

结论:

  • 阿普塔默显示粒子为SARS-CoV-2检测提供了一个多功能平台.
  • 开发的基于AdP的试验具有敏感性,选择性和潜在的低成本.
  • 这些发现为病毒流行病的快速诊断技术带来了有希望的进步.