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检测SARS-CoV-2尖端蛋白的检测特征使用Aptamer功能化的Si基电解质门式场效应晶体管 (EGT)

Seonghwan Shin¹, Sangwon Kim², Wonyeong Choi¹

¹Department of Electrical Engineering, Pohang University of Science and Technology (POSTECH), Pohang 37673, Republic of Korea.

|March 27, 2024

概括

这项研究表明,基于的电解质导入晶体管 (EGT) 可以检测高灵敏度的SARS-CoV-2尖端蛋白. 开发的aptamer功能化的EGT为快速准确的病毒检测提供了有前途的方法.

科学领域:

纳米技术和生物传感技术
材料科学材料科学材料科学
生物物理学的生物物理.

背景情况:

准确和敏感地检测SARS-CoV-2尖端蛋白对于疾病诊断和监测至关重要.
电解质通道晶体管 (EGT) 提供了一个潜在的平台,因为他们的高灵敏度,无标签的生物传感.
用特定的生物受体对EGT的功能化是实现针对性检测病毒蛋白的关键.

研究的目的:

为了研究上下制造的基于的EGT用于SARS-CoV-2尖端蛋白检测的传感性能.
为了评估EGT的灵敏度和检测极限 (LOD).
使用一次性容量模型分析传感机制.

主要方法:

制造基于的电解质通道晶体管 (EGT).
作为SARS-CoV-2尖端蛋白的特定受体的aptamer的EGT的功能化.
在子值和线性模式下对EGT的电气特性.
分析传感数据使用一次性容量模型结合二极电位和功能化层电容.

主要成果:

与线性方案相比,EGT在子值方案中表现出卓越的内在特性和更高的灵敏度.
达到检测极限 (LOD),电流灵敏度低至0.94 pg/mL,电压灵敏度为20 pg/mL.
一个集积容量模型有效地解释了传感响应,考虑到功能化层的有效二极极电位和电容.
亚胺功能化的EGT即使在10mM酸盐缓冲盐水 (PBS) 溶液中也表现出高灵敏度.

结论:

基于的EGT与体功能化是高度敏感和有效的检测SARS-CoV-2尖端蛋白.
EGT的子值制度为生物传感应用提供了更高的灵敏度.
这些发现突出了基于Si的EGT的潜力,作为开发用于病毒检测的先进诊断工具的有希望的平台.

关键词:

生物肥料和料 (BioFET) 是一种生物肥料和料.在 COVID-19 疫情中,美国欧洲欧洲欧洲欧洲欧洲这就是SARS-CoV-2病毒.一个aptamer的应用程序.

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