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在暴露于405nm激光光的Candida菌株中,红色/色自光

Rafał Wiench¹, Dariusz Paliga², Anna Mertas³

¹Department of Periodontal Diseases and Oral Mucosa Diseases, Faculty of Medical Sciences in Zabrze, Medical University of Silesia, 40-055 Katowice, Poland.

Dentistry journal

|March 27, 2024

概括

大多数Candida菌株在405nm激光下表现出红色/色自发光 (R/OF). 这一发现可能会为Candida spp.提供新的牙科查方法. 检测. 检测. 在检测.

科学领域:

医学真菌学医学真菌学
生物光子学生物光子学
诊断微生物学诊断微生物学

背景情况:

坎迪达菌种是重要的病原体,特别是在免疫受损的个体中.
这些真菌可以导致粘膜殖民和侵入性感染.
病原性真菌,包括Candida,经常显示红色/色自光 (R/OF),这是一种有用的检测特性.

研究的目的:

研究使用405nm二极管激光来观察口腔Candida物种中的R/OF.
探索R/OF检测在健康查中的潜力.

主要方法:

在Candida spp.的培养中. 在Sabouraud dextrose agar上生长.
Qdot 655 和 685 用于光基准测试.
一个405nm二极管激光照亮了培养物,并用黄色镜相机捕获图像.
视觉和计算分析评估了R/OF的存在,强度,色彩和变化.

主要成果:

大多数Candida菌株在405nm激光激发下表现出R/OF.
自体光在色彩和殖民地内部分布方面各不相同.
R/OF强度最初增加,然后随着时间的推移稳定下来.

结论:

大多数测试的Candida菌株在接触405nm激光时发出R/OF.
这一特征表明,可以将R/OF检测整合到常规牙科查中.
在口腔卫生环境中,R/OF成像为识别Candida物种提供了一种新的方法.

关键词:

自动光是一种自发光.二极管激光器二极管激光器化物化剂的使用方法口腔候群症的治疗方法波尔菲林斯 (porphyrins) 是一种有机物.这是一种原形氨酸 (protoporphyrin).酵母酵母是一种酵母.

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相关概念视频

Immunofluorescence Microscopy

Immunofluorescence Microscopy

A fluorescence microscope uses fluorescent chromophores called fluorochromes, which can absorb energy from a light source and then emit this energy as visible light. Fluorochromes include naturally fluorescent substances (such as chlorophylls) and fluorescent stains that are added to the specimen to create contrast. Dyes such as Texas red and FITC are examples of fluorochromes. Other examples include the nucleic acid dyes 4’,6’-diamidino-2-phenylindole (DAPI), and acridine orange.