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通过转录基因组集成基因组规模建模,Cordyceps militaris的曝光代谢反应

Panyawarin Soommat¹, Nachon Raethong², Ratchaprapa Ruengsang³

¹Genetic Engineering and Bioinformatics Program, Graduate School, Kasetsart University, Bangkok 10900, Thailand.

|March 27, 2024

概括

这项研究将转录组数据与基因组规模代谢模型 (GSMM) 整合在一起,以揭示光如何影响Cordyceps militaris代谢. 改进后的模型准确地预测了新陈代谢的变化,特别是在cordycepin和carotenoid的生产.

科学领域:

生物技术是生物技术.
代谢工程是代谢工程.
菌类学菌类学是指菌类学.

背景情况:

基因组规模代谢模型 (GSMM) 提供了对微生物代谢的洞察力.
标准的GSMM在预测对光等环境因素的反应方面存在局限性.
在不同的光线条件下,Cordyceps militaris的新陈代谢需要进一步调查.

研究的目的:

为Cordyceps militaris开发一种增强的GSMM,其中包括依赖光的代谢反应.
使用转录基因数据研究C. militaris适应光线所涉及的代谢途径.
提高GSMM的预测能力,以了解真菌代谢.

主要方法:

将转录组数据与Cordyceps militaris.的基因组规模代谢模型 (GSMM) 的整合.
通过代谢和表达 (GIMME) 调节的基因不活性框架的应用.
转录组集成的GSMM (tiGSMM) 模型的开发,iPS1474-tiGSMM.

主要成果:

该iPS1474-tiGSMM模型成功地模拟了C. militaris在暴露于光线下的代谢反应.
该模型表明,它可以更好地预测与表达基因相关的代谢流,这些基因在cordycepin和胡卜素生物合成中表达.
报告员的代谢物分析确定了中央碳,纯氨酸和脂肪酸代谢作为光照下胡卜素生产的关键过程.

结论:

转录组集成的GSMM为研究真菌中介光代谢调节提供了一种强大的方法.
确定了参与C. militaris适应光线条件的关键代谢途径.
这项研究提供了通过操纵代谢基因和流动来优化C. militaris中的代谢物产生的见解.

关键词:

科尔迪塞普斯军用菌 (Cordyceps militaris) 是一种植物.灯光灯光灯光灯光灯光代谢过程中的代谢.系统生物学系统生物学转录基因组集成的GSMM (tiGSMM) 是一个

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