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生物膜由Mycobacterium avium开发复杂的临床分离物:克拉里思罗米对超结构的影响
Arij Akir1, Abrar Senhaji-Kacha1,2, Maria Carmen Muñoz-Egea1,2
1Department of Clinical Microbiology, IIS-Fundación Jiménez Díaz, Universidad Autónoma de Madrid, 28040 Madrid, Spain.
Antibiotics (Basel, Switzerland)
|March 27, 2024
概括
所有Mycobacterium avium复杂菌株形成生物膜,具有每个菌株独特的特征,而不是物种或临床意义. 克拉里思罗米辛对这些生物膜的疗效取决于生物膜的年龄和治疗持续时间,显示了菌株特定的结果.
科学领域:
- 微生物学 微生物学
- 传染性疾病 传染性疾病
- 生物膜研究 生物膜研究
背景情况:
- 菌复合体 (MAC) 包含常见的非结核性菌,导致人类感染.
- 生物膜形成经常与MAC感染有关,但对MAC生物膜的研究有限.
研究的目的:
- 为了研究Mycobacterium avium复杂菌株的生物膜形成和澄素敏感性.
- 确定生物膜超结构或抗生素反应是否与MAC物种或临床意义相关.
主要方法:
- 使用聚焦激光扫描显微镜和特定染料检查MAC菌株的生物膜超结构.
- 评估了生物膜中的细菌活力以及克拉里胺在时间上的影响.
- 使用Kruskal-Wallis和Spearman的等级相关性测试进行统计分析.
主要成果:
- 在生物膜参数中观察到的菌株特异性差异,与物种或临床意义无关.
- 克拉里思罗米辛的疗效与生物膜年龄成反比例,除了两种M. chimaera菌株外.
- 即使在长时间暴露于澄胺,生物膜活力仍然显著 (≥40%).
结论:
- 所有研究的MAC菌株都形成具有菌株特征的生物膜.
- 克拉里思罗米对MAC生物膜的影响取决于生物膜年龄和治疗持续时间,是菌株特异性的,与临床意义无关.
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