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Karolina Banyś¹, Małgorzata Jelińska¹, Małgorzata Wrzosek²

¹Department of Toxicology and Food Science, Faculty of Pharmacy, Medical University of Warsaw, Poland, Banacha 1, 02-097 Warsaw, Poland.

Current issues in molecular biology

|March 27, 2024

概括

多种形式的基因斯坦补充剂在老鼠中加剧了瘤过程. 然而,它降低了特定的eicosanoids,同时增加了矩阵金属蛋白酶-9 (MMP-9) 水平和基因表达,影响了癌症的发展.

科学领域:

生物化学生物化学
在瘤学瘤学.
药理学药理学是指药理学的学科.

背景情况:

基因斯坦是一种大豆异黄,已显示出潜在的抗癌性质.
了解基尼斯坦的剂量依赖性和大小依赖性影响对于其治疗应用至关重要.
在瘤进展中,eicosanoids和促炎性细胞因子的作用需要进一步阐明.

研究的目的:

评估纳米,微和宏基因斯坦对大鼠DMBA诱导瘤的影响.
调查潜在的机制,包括对eicosanoids,细胞因子和MMP-9的影响.
评估基因斯坦对瘤强度和相关生物标志物的影响.

主要方法:

雌性Sprague Dawley大鼠 (n=32) 被分为四组:对照组和补充Genistein的 (宏观,微观,纳米).
乳腺腺癌被诱导使用7,12-二甲基-1,2-[a]炭烯 (DMBA).
使用液体染色学,免疫酶分析和qRT-PCR来量化埃可索诺因,细胞因子 (IL-1,IL-6) 和MMP-9.

主要成果:

基因斯坦补充剂,无论形式如何,都加剧了瘤过程.
补充组显示12-HEPE,HODE和12-HETE的水平明显较低.
纳米,微和宏基因斯坦增加了MMP-9水平,在宏基因组中显著增加了MMP-9基因表达.

结论:

在所有测试的形式中,饮食中的基因斯坦在老鼠中加剧了瘤过程.
基尼斯补充剂改变了eicosanoid配置文件和上调的MMP-9,这表明在癌症进展中起着复杂的作用.
这些发现凸显了在癌症研究和治疗中需要仔细考虑基因斯坦的剂量和形式的必要性.

关键词:

癌症癌症癌症癌症癌症基因斯坦 (genistein) 是一种植物.纳米颗粒是一种纳米粒子.

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