在兴奋剂控制中使用11-测作为氨 (11OXO) 管理的目标分析物
Thomas Piper1, Gregor Fußhöller1, Mario Thevis1,2
1Center for Preventive Doping Research, Institute of Biochemistry, German Sport University Cologne, Am Sportpark Müngersdorf 6, 50933 Köln, Germany.
Metabolites
|March 27, 2024
概括
在体育运动中,通过测量其代谢物11-oxo-testosterone (KT) 来检测氨 (11OXO) 兴奋剂. 尿样中KT的新值提高了检测准确度,减少了反兴奋剂工作中的假阳性.
科学领域:
- 运动科学 运动科学 运动科学
- 分析化学 分析化学
- 生物化学 生物化学
背景情况:
- 氨 (11OXO) 被世界反兴奋剂机构禁止在运动中使用.
- 目前的11OXO检测方法依赖于其代谢物,但初步值可能会由于自然变异导致错误阳性.
- 11-oxo-testosterone (KT) 是11OXO的代谢物,其检测和碳同位素比率 (CIR) 对抗兴奋剂至关重要.
研究的目的:
- 通过重新评估其代谢物KT的尿度值来完善11OXO的检测.
- 通过确定可靠的KT度和CIR值来提高反兴奋剂测试的准确性.
- 排除具有自然升高KT水平的样本,从而最大限度地减少假阳性.
主要方法:
- 对5232个常规兴奋剂检查样本的分析,以确定KT尿度的参考人群.
- 使用气相色谱与三重四极质谱相结合的尿路类固醇的量化.
- 碳同位素比率 (CIRs) 对于具有KT水平升高的106个样本的子集的研究,使用气相色谱与同位素比率质谱学相结合.
主要成果:
- 确定了新的KT尿度值为130 ng/mL.
- 这项研究成功地区分了自然发生的KT水平和由11OXO或KT给药引起的KT水平.
- 在证实非法物质使用和排除可疑发现时,CIR分析被证明是有效的.
结论:
- 建议的KT尿度值为130 ng/mL,提高了11OXO的反兴奋剂测试的可靠性.
- 尿度和CIR分析的综合方法为检测11OXO给药提供了可靠的方法.
- 这些发现有助于在体育领域制定更准确,更公平的反兴奋剂战略.
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