兰地利和蒂亚美索克萨姆暴露改变了与科罗拉多土豆虫中小型非编码RNA处理相关的转录的表达
Pierre Bastarache1, Kenan Timani1, Mariem Ben Youssef1
1Department of Chemistry and Biochemistry, Universite de Moncton, 18 Antonine-Maillet Avenue, Moncton, NB E1A 3E9, Canada.
Insects
|March 27, 2024
概括
科罗拉多马甲虫通过改变的小RNA通路发展出抗昆虫剂的抵抗力. 阿戈1转录下调减少了生存率,这表明它是.
科学领域:
- 昆虫学 昆虫学是一门学科.
- 分子生物学分子生物学
- 生物化学 生化学
背景情况:
- 科罗拉多马甲虫 (Leptinotarsa decemlineata) 是一种主要的农业害虫,以其快速抗虫剂的耐药性而闻名.
- 抗虫剂耐药性涉及复杂的分子机制,包括小非编码RNA通路的改变.
- 调节小RNA合成和功能的基因至关重要,但在抗虫剂耐药性方面并非完全具有特征.
研究的目的:
- 为了研究在L.L.中关键的小非编码RNA处理转录的表达. 暴露于兰利和硫胺的生物.
- 确定这些杀虫剂调节的特定转录和它们在甲虫反应中的作用.
- 评估Ago1的潜力,作为一个目标,以管理抗生素的耐药性.
主要方法:
- 使用qRT-PCR对Ago1,Ago2,Ago3,Dcr2a,Dcr2b,Expo-5,Siwi-1和Siwi-2的转录水平进行量化.
- 成人的治疗L.L. 在不同剂量和持续时间的西安特拉尼利普罗尔和蒂亚美索克萨姆下降.
- 使用dsRNA降低Ago1表达的RNA干扰 (RNAi).随后进行杀虫剂处理.
主要成果:
- 兰利露暴露改变了Ago1和Dcr2b转录水平.
- 蒂亚美索克萨姆暴露调节了Dcr2a和Siwi-1转录水平.
- 通过dSRNA对Ago1进行下调,然后进行兰利治疗,显著降低了昆虫的存活率.
结论:
- 杀虫剂差异调节涉及小非编码RNA处理的转录在L. 它们的数量正在下降.
- 阿戈1在对兰利的反应中起着至关重要的作用.
- 阿戈1代表了开发针对抗杀虫剂耐药的科罗拉多马甲虫的新战略的潜在目标.
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