磁性分子打印聚合物用于从料中选择性提取亚毒素
María Del Carmen Pérez-Álvarez1, Natalia Arroyo-Manzanares1, Natalia Campillo1
1Department of Analytical Chemistry, Faculty of Chemistry, Regional Campus of International Excellence "Campus Mare Nostrum", University of Murcia, 30100 Murcia, Spain.
Toxins
|March 27, 2024
概括
磁性分子印记聚合物 (MMIPs) 提供了一种用于检测猪料中的偏毒素的新方法. 这种技术简化了样品的准备,并准确地量化了这些有害污染物.
科学领域:
- 分析化学 分析化学
- 材料科学 材料科学 材料科学
背景情况:
- 毒素,特别是亚毒素,由于其毒性和经济影响,对动物健康和食品工业构成重大风险.
- 在动物料中检测和量化真菌毒素的有效方法对于确保食品安全和监管合规至关重要.
研究的目的:
- 开发和验证一种简化的样本制备方法,用于用磁性分子印记聚合物 (MMIP) 确定猪料中四种主要的亚毒素 (B 1 ,B 2 ,G 1和G 2).
- 评估基于MMIP的方法的效率和可靠性,用于对猪料等复杂矩阵中的亚毒素进行例行分析.
主要方法:
- 磁性分子印记聚合物 (MMIP) 通过将磁性纳米粒子 (Fe3O4) 与商业分子印记聚合物集成来制备,简化了合成过程.
- 使用光检测 (HPLC-FLD) 的高性能液体染色学用于对亚毒素的定量分析.
- 方法验证包括对量化极限 (LOQs),可重复性,中间精度和准确性的评估.
主要成果:
- 开发的MMIP方法实现了亚毒素的低LOQ (0.090.47 ng/g),低于欧盟监管限制.
- 证明了出色的精度,相对标准偏差低于10%的可重复性和中间精度.
- 准确度值在92%至111%之间,表明准确度很高.
- 该方法成功地应用于31个真实猪料样本,检测到的 aflatoxin度在0.2至3.2 ng/g之间.
结论:
- 拟议的基于MMIP的方法提供了一种简单,高效和准确的方法,用于选择性提取和确定猪料中的亚毒素.
- 这种创新材料将磁分离与分子印记相结合,为农业食品部门的真菌毒素分析提供了一个有前途的工具.
- 经过验证的方法符合监管要求,并证明在动物料中常规监测黄毒素的实际适用性.
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