通过固态微提取与液态染色学-质谱学相结合的人类唾液的蛋白质组分析
Runshan W Jiang1, Lina M Marin2, Karol Jaroch1,3
1Department of Chemistry, University of Waterloo, Waterloo, Ontario N2L 3G1, Canada.
Analytical chemistry
|March 27, 2024
概括
研究人员开发了生物相容的固体相微提取 (生物-SPME) 用于人类唾液蛋白质组. 这种新的方法成功识别了和蛋白质,区分了健康和SARS-CoV-2阳性个体.
科学领域:
- 生物化学 生物化学
- 分析化学 分析化学
- 生物医学科学 生物医学科学
背景情况:
- 人的唾液是用于非侵入性诊断的有价值的生物流体.
- 传统的固相微提取 (SPME) 方法在唾液提取方面存在局限性.
- 提取技术的进步对于唾液蛋白质组学至关重要.
研究的目的:
- 开发和验证一种新型生物相容的固相微提取 (生物-SPME) 技术,用于人类唾液蛋白质组学.
- 为了比较开发的生物SPME与商业SPME设备的效率.
- 在区分健康和SARS-CoV-2阳性个体时,应用生物-SPME-LC-MS/MS策略用于非向皮体.
主要方法:
- 开发多孔C18/多烯 (PAN) SPME刀片,其中包含一个用于增强提取的原体.
- 使用生物-SPME设备 (高达3.0kDa) 从人类唾液样本中提取的优化.
- 通过SPME-液体色谱-质谱/质谱 (SPME-LC-MS/MS) 分析素消化后提取的.
主要成果:
- 与商业SPME刀片相比,开发的生物-SPME刀片显示出更高的提取效率.
- 在使用生物-SPME.使用的所有分析的唾液样本中,一致确定了七种内源性蛋白质.
- 从健康和SARS-CoV-2阳性个体的唾液样本之间观察到显著的型差异.
结论:
- 生物-SPME-LC-MS/MS技术代表了人类唾液蛋白质组和基组的重大进步.
- 这种方法提高了提取低分子量的灵敏度和能力.
- 该研究强调了生物-SPME在生物医学应用中的潜力,包括疾病生物标志物发现.
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