亚甘酸激活转录因子模块MdABI5-MdMYBS1在由胡卜素衍生的果果色彩过程中
Dongjie Jia1,2, Yuchen Li1,2, Kun Jia1,2
1College of Horticulture, Qingdao Agricultural University, Qingdao 266109, China.
Plant physiology
|March 27, 2024
概括
果的颜色是由胡卜素和酸 (ABA) 调节的. 一个关键的转录因子MdMYBS1控制着胡卜素合成和ABA积累,受MdABI5和ABA本身的影响,影响水果质量.
科学领域:
- 植物生物学 植物生物学
- 分子遗传学 分子遗传学
- 生物化学 生物化学
背景情况:
- 胡卜素是果实颜色的关键颜料在果 (Malus domestica Borkh. ) 的情况.
- 之前的研究指出果突变种的颜色差异,但调节机制尚不清楚.
- 胡卜素生物合成及其调节对于水果的外观和质量至关重要.
研究的目的:
- 阐明果中由胡卜素衍生的水果颜色背后的调节机制.
- 为了确定关键的基因和转录因子,参与胡卜素和酸 (ABA) 代谢.
- 了解胡卜素,ABA和水果颜色之间的相互作用.
主要方法:
- 识别和表征R1型MYB转录因子.
- 对MdMYBS1.1.的基因表达分析 (过度表达和沉默)
- 对果果中的胡卜素和ABA含量的分析.
- 促进子活动测定目标基因和转录因子.
- 调查MdABI5和ABA在监管途径中的作用.
主要成果:
- 确定了MdMYBS1,一种R1型MYB转录因子,并且与胡卜素和ABA水平密切相关.
- 过度表达MdMYBS1增加,而沉默减少,胡卜素和ABA积累.
- MdMYBS1直接激活了关键的胡卜素生物合成基因 (MdPSY2-1,MdLCYb) 和ABA生物合成基因 (MdNCED1).
- MdABI5 作为 MdMYBS1.1. 的上游激活剂.
- ABA积极调节胡卜素生物合成,并增强MdMYBS1和MdABI5.5的促进活性.
结论:
- 由ABA激活的MdABI5-MdMYBS1调节级联,控制着由胡卜素衍生的水果颜色和果中的ABA积累.
- 这一途径为果的繁殖和种植策略提供了洞察力,以增强果的颜色和质量.
- 了解这种分子机制是改善果园艺特征的关键.
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