激活GTPase的蛋白质Gyp7调节了Rab7/Ypt7在晚期内分泌体上的活性
Nadia Füllbrunn1,2, Raffaele Nicastro3, Muriel Mari4
1Department of Biology/Chemistry, Biochemistry Section, Osnabrück University, Osnabrück, Germany.
The Journal of cell biology
|March 27, 2024
概括
GTPase激活蛋白Gyp7对于酵母真空细胞形态和内分泌体运输至关重要. 它对晚期内分泌体的特定定位对于调节Ypt7活动和TORC1信号传递至关重要.
科学领域:
- 细胞生物学 细胞生物学
- 分子生物学分子生物学
- 生物化学 生物化学
背景情况:
- 拉布GTPases在内膜系统中作为器官识别标记物起作用.
- 关氨酸核酸交换因子 (GEFs) 和GTPase激活蛋白 (GAPs) 调节Rab GTPase的局部化和活性.
- 对GEF和GAP有机体向和活动监管的机制在很大程度上仍然没有特征.
研究的目的:
- 研究酵母中的GTPase激活蛋白Gyp7的细胞器向和功能.
- 阐明Gyp7在调节Ypt7GTPase中的作用及其对真空细胞形态和内分泌体运输的影响.
- 探索Gyp7膜结合和活性所需的脂质.
主要方法:
- 显微镜观察Gyp7定位和真空球形态.
- 基因操纵以诱导Gyp7的错误准.
- 对于Gyp7的体外脂质结合和活性试验.
- 对内分泌体运输和TORC1信号通路的分析.
主要成果:
- 令人惊的是,Gyp7仅在酵母真空球附近的点点中观察到.
- 将Gyp7误导到真空细胞中,损害了真空细胞形态和内分泌体运输.
- Gyp7显示了对膜结合和GTPase激活蛋白活性的特定脂质要求.
- 过度表达Gyp7导致Ypt7度在晚期内分泌体和拉巴胺素耐药性,表明在内分泌体信号传递中发挥了作用.
结论:
- 在调节Ypt7.7的功能方面,Gyp7的内体位点对其功能至关重要.
- Gyp7对于有效的内分泌体运输和可能在信号内分泌体中的功能至关重要.
- 建议Gyp7成为管理晚期内分泌体功能的调节机制的一部分.
更多相关视频
11:05Analysis of Endocytic Uptake and Retrograde Transport to the Trans-Golgi Network Using Functionalized Nanobodies in Cultured Cells
Published on: February 21, 2019
9.0K
13:51Detection of Small GTPase Prenylation and GTP Binding Using Membrane Fractionation and GTPase-linked Immunosorbent Assay
Published on: November 11, 2018
9.9K
相关概念视频
Rab Proteins
3.9K
Rab proteins constitute the largest family of monomeric GTPases, of which 70 members are present in humans. Rab proteins and their effectors regulate consecutive stages of vesicle transport such as vesicle transport, docking, and fusion to the correct recipient membrane.
Rab proteins switch between a cytosolic, GDP-bound inactive state and a membrane-anchored, GTP-bound active state. By themselves, Rabs show slow rates of GDP/GTP exchange and GTP hydrolysis. Thus, Rab proteins are considered...
Rab proteins switch between a cytosolic, GDP-bound inactive state and a membrane-anchored, GTP-bound active state. By themselves, Rabs show slow rates of GDP/GTP exchange and GTP hydrolysis. Thus, Rab proteins are considered...
3.9K
Rab Cascades
2.6K
Rab GTPases act in a regulated cascade during membrane fusion, helping the lipid bilayers mix. The Rab family of proteins are active when bound to GTP, and inactive when bound to GDP. Hence, they act as guanine nucleotide-dependent molecular switches. Rab-GTP recognizes and binds to long or short-range tethering proteins to capture the target vesicle. These tethers coordinate with SNAREs on the vesicle and the target membrane to assemble the trans SNARE complex that locks the mixing bilayers.
2.6K
Coat Assembly and GTPases
3.5K
Vesicles incorporate different coat protein subunits in different cell locations, which changes the properties of the coat, such as the shape and geometry of the transport vesicles. Thus, vesicle coat proteins also play a significant role in cargo selection.
Coat assembly depends on the local availability of phosphatidylinositol phosphates or PIPs and GTP-binding proteins. Adaptor proteins, which link the coat proteins to the membrane, bind to these PIPs and play a crucial role in controlling...
Coat assembly depends on the local availability of phosphatidylinositol phosphates or PIPs and GTP-binding proteins. Adaptor proteins, which link the coat proteins to the membrane, bind to these PIPs and play a crucial role in controlling...
3.5K
Small GTPases - Ras and Rho
3.9K
Ras and Rho are small monomeric GTPases that act downstream of receptor tyrosine kinase (RTK) and regulate various cellular processes. These GTPases switch between active and inactive states by binding to guanine nucleotides.
Three regulatory proteins control their activity:
Three regulatory proteins control their activity:
3.9K
GTPases and their Regulation
8.4K
Guanine nucleotide-binding proteins (G-proteins), also known as GTPases, are a superfamily of proteins that regulate many cellular processes, such as cell signaling, vesicular transport, and the regulation of cell shape and motility. Mutation or dysfunction of these proteins can lead to disease. There are around 40,000 known G-proteins that can broadly be classified into two groups ‒ small G-proteins consisting of a single domain and large multi-domain G-proteins.
Large G-proteins,...
Large G-proteins,...
8.4K
The Early Endosome: Endocytosis of Transferrin
3.3K
Essential proteins such as insulin or low-density lipoprotein (LDL) and micronutrients such as iron enter a eukaryotic cell through receptor-mediated endocytosis. Subsequently, the early endosomes fuse with the vesicles containing such receptor-ligand complexes and play a vital role in sorting the incoming ligands and receptors. While the ligands are either degraded inside the vesicle or released into the cytosol, their receptors are returned to the plasma membrane for further rounds of...
3.3K
