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Updated: Aug 3, 2026

Rapid Genotyping of Animals Followed by Establishing Primary Cultures of Brain Neurons
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Rapid Genotyping of Animals Followed by Establishing Primary Cultures of Brain Neurons

Published on: January 29, 2015

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通过测序来检测转化酶可访问的染色质的代试验,以分离功能相关的神经元亚型

Collin B Merrill1, Iris Titos1, Miguel A Pabon2

  • 1Department of Psychiatry, Huntsman Mental Health Institute, University of Utah, Salt Lake City, UT 84108, USA.

Science advances
|March 27, 2024
PubMed
概括

研究人员开发了一种新方法,使用对转化酶可访问色素 (ATAC) 的测定方法,精确地准Drosophila大脑中的特定神经元子集,用于基因操纵和研究.

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Single-cell RNA-Seq of Defined Subsets of Retinal Ganglion Cells

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Post-differentiation Replating of Human Pluripotent Stem Cell-derived Neurons for High-content Screening of Neuritogenesis and Synapse Maturation00:06

Post-differentiation Replating of Human Pluripotent Stem Cell-derived Neurons for High-content Screening of Neuritogenesis and Synapse Maturation

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相关概念视频

Southern Blot02:57

Southern Blot

Agarose gel electrophoresis is very useful in separating DNA fragments by size. Running a DNA ladder containing fragments of the known length alongside the sample helps determine the approximate length of the sample DNA fragments. However, additional steps are needed to verify the sequence identity of the sample DNA fragments.
Denatured DNA fragments must be transferred onto a carrier membrane from the gel to make it accessible to a probe - a small ssDNA fragment complementary to the target DNA...