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Updated: Jun 29, 2025

Probe-based Real-time PCR Approaches for Quantitative Measurement of microRNAs
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Probe-based Real-time PCR Approaches for Quantitative Measurement of microRNAs

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一个,一个步骤,无标签的miRNA检测方法基于贝探头结构转换的结构转换

Jae Hoon Jeung1, Hyogu Han2, Se Hee Jang3

  • 1Material & Component Convergence R&D Department, Korea Institute of Industrial Technology (KITECH), Ansan, Republic of Korea; Department of Biological Engineering, College of Engineering, Konkuk University, Seoul, 05029, Republic of Korea.

Talanta
|March 27, 2024
PubMed
概括

这项研究引入了一种新的,无标签的方法,用于检测微RNA (miRNA),使用子探针的结构过渡. 这种方法使敏感和快速的miRNA识别成为可能,适合于临床诊断.

关键词:
生物传感器是一种生物传感器.头探测器探测器亮起的RNA吸收酶的亮起滚动圆转录 (RCT) 是一种结构转型 结构转型这是一个小RNARNA.

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Labeling DNA Probes03:31

Labeling DNA Probes

DNA probes are fragments of DNA labeled with a reporter tag to enable their detection or purification. The resulting labeled DNA probes can then hybridize to target nucleic acid sequences through complementary base-pairing, and may be used to recover or identify these regions.
Radioisotopes, fluorophores, or small molecule binding partners like biotin or digoxigenin, are the most widely used reporter tags for labeling DNA probes. These labels can be attached to the probe DNA molecule via...
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