在西班牙裔年轻人中暴露于per-和多基基物质和高通量蛋白质组
Jiawen Carmen Chen1, Jesse A Goodrich1, Douglas I Walker2
1Department of Population and Public Health Sciences, University of Southern California Keck School of Medicine, Los Angeles, CA, United States.
Environment international
|March 27, 2024
概括
和多醇基物质 (PFAS) 暴露与西班牙裔年轻人中蛋白质配置的改变有关,这表明潜在的炎症和免疫失调的分子机制. 这项研究揭示了PFAS影响的关键生物途径. 关键词:PFAS,蛋白质组学,心脏代谢,炎症,免疫调节.
科学领域:
- 环境健康科学 环境健康科学
- 蛋白质组学是指蛋白质组学.
- 心脏代谢健康状况
背景情况:
- 流行病学研究将和多醇基物质 (PFAS) 暴露与糖尿病和高血压等不良心脏代谢结果联系起来.
- 在人类中,PFAS诱导的心脏代谢作用背后的特定生物机制尚不清楚.
研究的目的:
- 在西班牙裔青少年和年轻成年人中调查暴露于特定PFAS (PFOS,PFOA,PFHxS,PFHpS,PFNA) 和血蛋白质谱之间的关联.
- 通过使用高通量蛋白质组学来探索受 PFAS 暴露影响的生物通路和分子功能.
主要方法:
- 分析了来自312名青少年 (SOLAR队列) 和137名年轻成年人的血样本 (Meta-AIR队列).
- 使用液态染色学高分辨率质谱学量化五个关键的PFAS.
- 使用Olink Explore Cardiometabolic Panel I和近距离延伸试验测量了334种血蛋白.
主要成果:
- 在两个队伍中,在PFAS暴露和参与炎症,免疫力和氧化应激的蛋白质之间发现了显著的关联.
- 在SOLAR队列中,PFNA与多种蛋白质的关联最强,而在Meta-AIR队列中,PFAS混合物与GDF15和IL6有关.
- 途径分析表明PFAS与免疫相关和炎症途径的激活,以及与免疫调节相互作用和JAK-STAT信号传递相关的常见蛋白质集群.
结论:
- 暴露于PFAS与蛋白质组状况的广泛变化有关,特别是与前炎症和免疫调节相关的变化.
- 这些蛋白质变化为推动PFAS毒性和心脏代谢干扰的潜在分子机制提供了洞察力.
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