pH 取决于HSF1三分化是由分子内相互作用塑造的
Bo-Hee Choi1, Chang-Ju Lee1, Tae Hwan Kim1
1Department of Chemistry and Chemistry Institute of Functional Materials, Pusan National University, Busan, 609-735, South Korea.
Biochemical and biophysical research communications
|March 27, 2024
概括
热冲击因子1 (HSF1) 三度化受pH的调节. 一个特定的残留物 (103) 影响最佳pH值,影响细胞应激反应.
科学领域:
- 分子生物学分子生物学
- 细胞生物学 细胞生物学
- 生物化学 生物化学
背景情况:
- 热冲击因子1 (HSF1) 对于细胞应激反应至关重要.
- 以前的研究表明,低生理pH值在体外激活HSF1,但机制尚不清楚.
研究的目的:
- 通过pH阐明HSF1激活的分子机制.
- 为了研究HSF1三元化对pH值变化的作用.
主要方法:
- 人类和金鱼HSF1.1的广泛突变发生.
- 在不同pH值下分析HSF1三元化.
- 调查残留物103在HSF1寡合化中的作用.
主要成果:
- 在HSF1的三元化过程中,pH的最佳值是由103的残留物决定的.
- 在残留103的氨酸显著增加了三元化最佳pH值.
- 由103残留物影响的His101和His110的质子状态通过与Cys36.6的相互作用来调节HSF1的三元化.
结论:
- 残留物103是HSF1pH依赖三分化的一个关键决定因素.
- 伊斯蒂丁质子化状态调节了分子内相互作用,改变了HSF1的寡合化.
- 这种机制可以了解HSF1在细胞pH恒温和应激反应中的作用.
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