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一种新的酶相关联联联体-吸收剂测定 (ELLSA) 用于查肺结核病:一项回顾性横截面研究

Gang Sheng¹, Hongqian Chu², Huijuan Duan³

¹Translational Medicine Center, Beijing Chest Hospital, Capital Medical University, Beijing 101149, China.

Microbes and infection

|March 27, 2024

概括

开发一种新型的酶相关联联体-吸收剂测定 (ELLSA) 用于在血清中检测肺结核 (PTB). 这种新方法显示了PTB的优异诊断性能,超过了现有的测试.

科学领域:

免疫学免疫学免疫学
生物化学生物化学
传染性疾病传染性疾病

背景情况:

对肺结核 (PTB) 患者血清中抗原存在的有限知识阻碍了诊断技术的开发.
在PTB血清中检测抗原具有改善诊断的巨大潜力.

研究的目的:

开发和评估使用血清生物标志物用于PTB的新型诊断试验.
在PTB患者血清中识别特定的人类配体和Mycobacterium结核病 (M.tb) 蛋白质.

主要方法:

在PTB血清中,使用人类蛋白质芯片和LC-MS/MS.识别了人类连接蛋白和M.tb蛋白.
针对C5orf24和M.tb蛋白分别制备了单克隆和多克隆抗体.
一种酶相关联联体-吸收剂测定 (ELLSA) 已开发并对412名志愿者 (250名PTB,162名健康对照) 进行了测试.

主要成果:

确定了9种特定于PTB患者的配体蛋白,其中C5orf24和基诺西林显示出高信号.
基于C5orf24的ELLSA在活跃PTB (ATB) 患者中显示了69.6%的灵敏度和90.18%的特异性.
在细菌阴性PTB中,ELLSA显示了64.22%的灵敏度,而MGIT 960和Xpert M.tb/RIF的灵敏度为0%.

结论:

血清中的M.tb蛋白可以通过连接蛋白有效丰富.
开发的ELLSA在PTB检测方面表现出卓越的诊断性能.

关键词:

吸附方式吸附方式吸附方式抗原检测检测抗原检测检测在C5orf2424中使用.连接物连接物连接物结核菌菌菌 (M.tb) 是一种

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