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基于生物信息学对关键LncRNAs的查,以调节与人体脊柱状网状细胞中与玻璃眼相关的转录组

Junhong Guo¹, Yunfei Wu², Yue Sun²

¹Shenzhen Eye Hospital, Jinan University, Shenzhen Eye Institute, 518040 Shenzhen, Guangdong, China.

Frontiers in bioscience (Landmark edition)

|March 27, 2024

概括

像SNHG8和EZH2这样的长非编码RNAs (lncRNAs) 合作调节人类带网状细胞中的基因表达,为青光眼治疗提供了新的治疗点.

科学领域:

眼科医生眼科眼科
分子生物学分子生物学
遗传学是一个遗传学.

背景情况:

玻璃眼与人体脊髓网 (HTM) 细胞的失调有关,但潜在的分子机制尚不清楚.
基质硬度增加是HTM细胞功能障碍的一个因素.
长非编码RNAs (lncRNAs) 是细胞过程的关键调节者.

研究的目的:

调查 lncRNAs 在 HTM 细胞对基质刚度增加的反应中的作用.
确定特定的lncRNA及其参与HTM调节的分子点.
为了探索眼的潜在治疗点.

主要方法:

对转录组测序 (RNA-seq) 数据的生物信息学分析,以识别失调的 lncRNAs.
功能性研究涉及HTM细胞中lncRNA表达干扰.
交叉链接免疫沉和测序 (CLIP-seq) 用于识别EZH2向RNAs.
染色体免疫沉和测序 (ChIP-seq) 以确定EZH2和H3K27me3的目标.

主要成果:

数以千计的lncRNA被确定为因基质刚度增加而失调.
lncRNA干扰证明了细胞外矩阵 (ECM) 相关基因的调节.
随着基质刚度的增加,EZH2表达减少.
SNHG8被确定为一种EZH2结合的lncRNA,在SNHG8调节基因中具有EZH2结合位.

结论:

SNHG8和EZH2通过影响HTM细胞中的RNA丰度来调节基因表达.
这种相互作用支持HTM细胞形态和密度.
鉴定到的lncRNAs,特别是SNHG8,提供了对HTM改变的洞察力,并提出了新的治疗策略.

关键词:

在 EZH2 中使用.在SNHG8中,SNHG8是SNHG8.玻璃眼 glaucoma 玻璃眼玻璃眼玻璃眼玻璃眼人类脑状网状组织细胞在 lncRNAs 中.

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