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Updated: Jun 29, 2025

Use of Frozen Tissue in the Comet Assay for the Evaluation of DNA Damage
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彗星测定作为一种评估冷保存样本中DNA损伤的方法

B P Plitta-Michalak1, A Ramos2, D Stepien3

  • 1Department of Chemistry, Faculty of Agriculture and Forestry, University of Warmia and Mazury in Olsztyn, Olsztyn, Poland. beata.plitta-michalak@uwm.edu.pl.

Cryo letters
|March 27, 2024
PubMed
概括

彗星测定是一种敏感的方法,用于评估细胞中的DNA损伤和修复. 它对于优化动物和植物研究中的冷保存协议至关重要,尽管在植物应用中存在挑战.

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Conventional electron microscopy (EM) involves dehydration, fixation, and staining of biological samples, which distorts the native state of biological molecules and results in several artifacts. Also, the high-energy electron beam damages the sample and makes it difficult to obtain high-resolution images. These issues can be addressed using cryo-EM, which uses frozen samples and gentler electron beams. The technique was developed by Jacques Dubochet, Joachim Frank, and Richard Henderson, for...
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