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基板 mesoSPIM:为清除样品的下一代开源光片显微镜

Nikita Vladimirov^1,2,3, Fabian F Voigt^4,5,6, Thomas Naert⁷

¹Brain Research Institute, University of Zurich, Zurich, Switzerland. vladimirov@hifo.uzh.ch.

Nature communications

|March 28, 2024

概括

下一代 mesoSPIM 显微镜为成像大型清除组织提供了增强的功能. 这种开源光片显微镜系统提供了更高的分辨率,更快的成像,并支持更大的样本用于各种科学应用.

科学领域:

生物物理学的生物物理.
光学工程是指光学工程.
生物成像技术生物成像技术

背景情况:

最初的 mesoSPIM 倡议 (2015) 旨在为大型清除组织进行光片显微镜的民主化.
对更大的生物样本进行高速,高分辨率成像的需求日益增加,需要先进的显微镜解决方案.

研究的目的:

介绍下一代的MesoSPIM (长板) 显微镜.
显著提高对大规模生物样本的成像能力.
提高光片显微镜的可访问性,可负担性和易于组装.

主要方法:

开发了一种新的光学方法,用于评估光板显微镜中的大传感器摄像头的检测目标.
设计了具有扩展视野和优化光学组件的"板" mesoSPIM.
集成功能可提高吞吐量,提高分辨率,并与各种组织清除技术兼容.

主要成果:

在整个视野中实现了1.5微米横向和3.3微米轴向的高空间分辨率.
启用了高达20×的放大效果,可以进行详细的成像.
支持的样本大小从亚毫米到几厘米不等.
证明与多种清算方法的兼容性.

结论:

"台式"mesoSPIM代表了对大型清除组织的可访问,高性能光板显微镜的重大进步.
这一下一代系统解决了越来越多地需要在各种生物尺度上进行详细的成像.
改进的 mesoSPIM 准备加速神经科学,发育生物学,病理学和物理学的研究.

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