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相关概念视频

Confocal Fluorescence Microscopy01:16

Confocal Fluorescence Microscopy

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Confocal microscopy is an advanced microscopic technique. The prime advantage of the confocal microscope over other microscopy techniques is its ability to block the out-of-focus light from the illuminated samples using pinholes. It is widely used with fluorescence optics to obtain high-resolution, sharp contrast images. Unlike optical microscopes, confocal microscopes use a focused beam of light laser to scan the entire sample surface at different z-planes. These microscopes are, therefore,...
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Imaging Biological Samples with Optical Microscopy01:18

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Optical microscopy uses optic principles to provide detailed images of samples. Antonie van Leeuwenhoek designed the first compound optical microscope in the 17th century to visualize blood cells, bacteria, and yeast cells. In 1830, Joseph Jackson Lister created an essentially modern light microscope. The 20th century saw the development of microscopes with enhanced magnification and resolution.
In optical microscopy, the specimen to be viewed is placed on a glass slide and clipped on the stage...
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The early pioneers of microscopy opened a window into the invisible world of microorganisms. In 1830, Joseph Jackson Lister created an essentially modern light microscope. The 20th century saw the development of microscopes that leveraged nonvisible light, such as fluorescence microscopy that uses an ultraviolet light source and electron microscopy that uses short-wavelength electron beams. These advances significantly improved magnification, image resolution, and contrast. By comparison, the...
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相关实验视频

Updated: Jun 29, 2025

Single Plane Illumination Module and Micro-capillary Approach for a Wide-field Microscope
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Single Plane Illumination Module and Micro-capillary Approach for a Wide-field Microscope

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基板 mesoSPIM:为清除样品的下一代开源光片显微镜.

Nikita Vladimirov1,2,3, Fabian F Voigt4,5,6, Thomas Naert7

  • 1Brain Research Institute, University of Zurich, Zurich, Switzerland. vladimirov@hifo.uzh.ch.

Nature communications
|March 28, 2024
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此摘要是机器生成的。

下一代 mesoSPIM 显微镜为成像大型清除组织提供了增强的功能. 这种开源光片显微镜系统提供了更高的分辨率,更快的成像,并支持更大的样本用于各种科学应用.

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相关实验视频

Last Updated: Jun 29, 2025

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科学领域:

  • 生物物理学的生物物理.
  • 光学工程是指光学工程.
  • 生物成像技术 生物成像技术

背景情况:

  • 最初的 mesoSPIM 倡议 (2015) 旨在为大型清除组织进行光片显微镜的民主化.
  • 对更大的生物样本进行高速,高分辨率成像的需求日益增加,需要先进的显微镜解决方案.

研究的目的:

  • 介绍下一代的MesoSPIM (长板) 显微镜.
  • 显著提高对大规模生物样本的成像能力.
  • 提高光片显微镜的可访问性,可负担性和易于组装.

主要方法:

  • 开发了一种新的光学方法,用于评估光板显微镜中的大传感器摄像头的检测目标.
  • 设计了具有扩展视野和优化光学组件的"板" mesoSPIM.
  • 集成功能可提高吞吐量,提高分辨率,并与各种组织清除技术兼容.

主要成果:

  • 在整个视野中实现了1.5微米横向和3.3微米轴向的高空间分辨率.
  • 启用了高达20×的放大效果,可以进行详细的成像.
  • 支持的样本大小从亚毫米到几厘米不等.
  • 证明与多种清算方法的兼容性.

结论:

  • "台式"mesoSPIM代表了对大型清除组织的可访问,高性能光板显微镜的重大进步.
  • 这一下一代系统解决了越来越多地需要在各种生物尺度上进行详细的成像.
  • 改进的 mesoSPIM 准备加速神经科学,发育生物学,病理学和物理学的研究.