从芝麻油粉中分离的细菌细菌SOM8,用于潜在的益生菌应用,抑制人类肠病原体
Zhongtian Zhao1, Wenrui Li1, The Thien Tran1
1School of Materials Science and Engineering, Nanyang Technological University, Singapore, Singapore.
BMC microbiology
|March 28, 2024
概括
从食物浪费中分离出来的 Bacillus subtilis SOM8 显示出作为益生菌的强大潜力. 它有效地向人类病原体,并显示出有利的安全概况,可用于进一步的临床开发.
科学领域:
- 微生物学 微生物学
- 益生菌研究 益生菌研究
- 食品科学 食品科学 食品科学
背景情况:
- 细菌物种被探索为益生菌,但由于某些菌株可能产生毒素,安全查至关重要.
- 确定安全有效的Bacillus益生菌菌株需要在临床应用之前进行严格的评估.
研究的目的:
- 从芝麻油中分离和评估潜在的益生菌菌株.
- 综合评估一个有前途的候选药物的安全性和有效性, Bacillus subtilis SOM8,针对人类肠道病原体.
主要方法:
- 使用状方法从SOM中分离出菌株.
- 进行了抗致病活性,应激耐受性 (酸性,胆盐,模拟液体,热量),与宿主相关的益处 (抗氧化剂,BSH活性),体外安全性 (血液溶解,细胞毒性),基因分析 (毒素基因,移动元素) 和生物活性化合物的生物信息预测.
主要成果:
- 隔离了9种具有抗病原性质的菌株; Bacillus subtilis SOM8显示了对5种人类肠病原体最有前途的活性.
- 细菌细菌SOM8表现出对各种压力因素的耐受性,具有抗氧化剂和胆盐酸酶活性,并表现出低细胞毒性.
- 安全性评估显示,只有血解毒素基因,部分血解,内在抗菌素耐药性,并没有等离子体. 生物信息分析预测了风素和脂素的存在.
结论:
- 细菌细菌SOM8表现出相当大的潜力作为一种益生菌针对人类肠病原体,基于其在选择试验中的强大表现.
- 现型和基因型安全评估表明,Bacillus subtilis SOM8.8的临床前安全概况是有利的.
- 细菌细菌SOM8是一个有前途的益生菌候选人,需要进一步的临床开发.
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