猪粒状细胞 - 细胞衍生外体增强卵细胞发育:一个体外研究
Jiajie Ren1,2, Yue Ding1,2, Junsong Shi3
1State Key Laboratory of Swine and Poultry Breeding Industry, National Engineering Research Center for Breeding Swine Industry, College of Animal Science, South China Agricultural University, Guangzhou 510000, China.
Antioxidants (Basel, Switzerland)
|March 28, 2024
概括
猪粒粉细胞衍生外体 (GC-EXs) 通过提高抗氧化能力和线粒体功能来增强卵细胞的发育. 这种效应是由针对DOCK6的miR-148a-3p调节的,突出了生殖生物学中的新型调节机制.
科学领域:
- 生殖生物学 生殖生物学
- 细胞生物学 细胞生物学
- 细胞外囊泡 细胞外囊泡
背景情况:
- 毛囊液衍生的外体促进卵细胞的发育,但它们的具体来源和调节作用尚不清楚.
- 卵细胞的发育依赖于花质细胞-卵细胞的交流,但花质细胞分泌的外体的功能仍然不太清楚.
研究的目的:
- 调查猪粒粉细胞衍生外体 (GC-EXs) 在调节卵细胞发育中的作用.
- 阐明GC-EXs对卵细胞质量影响的分子机制.
主要方法:
- 建立一个体外猪粒粉细胞模型,并收集/识别GC-EXs.
- 在体外培养的累积卵细胞复合物 (COCs) 补充GC-EXs.
- 小RNA测序,生物信息学分析,双 luciferase 记者基因测定和 RT-qPCR 来识别关键的 miRNA 和目标基因.
主要成果:
- 补充GC-EXs改善了累积膨胀指数 (CEI),减轻了活性氧物种 (ROS) 和增加了卵细胞中的线粒体膜潜力 (MMP).
- 小RNA测序确定了miR-148a-3p作为GC-EXs中的高度表达的miRNA.
- miR-148a-3p模仿者复制了GC-EXs的积极影响,并且DOCK6被确认为其向基因.
结论:
- 猪GC-EX通过增强抗氧化能力和线粒体功能,显著促进卵细胞的发育.
- 该机制涉及外体miR-148a-3p针对DOCK6,这表明在 oogenesis 中细胞间通信的新途径.
- 在辅助生殖技术中,GC-EXs是改善卵细胞质量的有前途的治疗剂.
相关概念视频
01:20Folliculogenesis
744
Folliculogenesis is the development of ovarian follicles, the specialized structures within the ovarian cortex where oogenesis, or egg development, occurs. This process is essential for female reproductive health and begins during fetal development when primordial follicles are formed. Each primordial follicle comprises a primary oocyte in the center, surrounded by a single layer of squamous pre-granulosa cells. These follicles remain dormant in late prophase I of meiosis until triggered by...
744
01:22Oogenesis
1.5K
Oogenesis, the process of developing egg cells (female gametes), occurs within the ovaries and is fundamental to female fertility. This sequence begins during fetal development when diploid oogonia in the developing ovaries undergo mitotic divisions to produce primary oocytes. By birth, these primary oocytes enter prophase I of meiosis but become arrested in this stage, remaining suspended until puberty.
Each primary oocyte is surrounded by a layer of pre-granulosa cells, forming what is...
Each primary oocyte is surrounded by a layer of pre-granulosa cells, forming what is...
1.5K