一种潜在的神经缩病毒 (NNV) 活疫苗,用于通过基因组修改获得的鱼
Lucía Vázquez-Salgado1, Sandra Souto1, José G Olveira1
1Instituto de Acuicultura, Departamento de Microbiología y Parasitología, Universidade de Santiago de Compostela, 15706 Santiago de Compostela, Spain.
Animals : an open access journal from MDPI
|March 28, 2024
概括
一种针对病毒性脑病变和视网膜病变 (VER) 的新型活疫苗显示出有前途. 这种神经缩病毒 (NNV) 候选疫苗可以提高鱼类的生存率,并增强对疾病的免疫反应.
科学领域:
- 水生动物卫生 水生动物卫生
- 病毒学 病毒学
- 免疫学 免疫学 免疫学
背景情况:
- 病毒性脑病和视网病 (VER) 是一种严重的鱼类神经系统疾病,由神经缩病毒 (NNV) 引起.
- 感染NNV导致在早期发育阶段显著的死亡率,影响水产养殖.
- 目前对VER的控制策略有限,需要开发疫苗.
研究的目的:
- 评估一种具有特定3'NCR突变的逆转基因RGNNV/SJNNV菌株,作为弱活体疫苗.
- 为了确认工程NNV菌株的衰减.
- 评估疫苗在保护鱼免受重量NNV感染方面的有效性及其对免疫系统的影响.
主要方法:
- 在22°C下用逆向工程RGNNV/SJNNV菌株对子进行实验性感染,以确认减弱.
- 在18°C和22°C使用浴,肌内和腹腔内途径进行疫苗接种试验.
- 对鱼类生存率,病毒复制和免疫相关基因表达 (cd4,cd8,tlr7,tnfα,rtp3,mx) 的分析.
主要成果:
- 工程NNV菌株被证实在豆中减弱.
- 与未接种疫苗的对照组相比,接种疫苗的鱼类的生存率明显提高.
- 疫苗接种导致病毒复制延迟和减少,并对关键免疫反应基因进行上调.
结论:
- 减弱的RGNNV/SJNNV菌株是针对VER的活体疫苗的一个有希望的候选人.
- 该疫苗有效地提高了单独的生存率,并引起了针对NNV感染的强有力的免疫反应.
- 这项研究为开发用于水产养殖的有效NNV疫苗提供了基础.
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