相关实验视频

Updated: Jun 29, 2025

Subcellular Fractionation of Primary Chronic Lymphocytic Leukemia Cells to Monitor Nuclear/Cytoplasmic Protein Trafficking

Subcellular Fractionation of Primary Chronic Lymphocytic Leukemia Cells to Monitor Nuclear/Cytoplasmic Protein Trafficking

Published on: October 23, 2019

对原发性慢性淋巴细胞白血病细胞信号通路的分析

Josipa Skelin¹, Maja Matulić², Lidija Milković¹

¹Ruđer Bošković Institute, 10000 Zagreb, Croatia.

|March 28, 2024

概括

慢性淋巴细胞白血病 (CLL) 的结果因活性基因和途径而异. 在实验室中NOTCH1通路的激活会增加CLL细胞亡,即使BCL2表达高.

科学领域:

血液学血液学血液学
分子生物学分子生物学
在瘤学瘤学.

背景情况:

慢性淋巴细胞白血病 (CLL) 是一种具有异质临床结果的B细胞恶性瘤.
疾病的进展与特定的基因和恶性克隆内的信号通路有关.
涉及的关键途径包括NOTCH1,Ikaros家族基因,BCL2和NF-κB,促进白血病细胞生存和增殖.

研究的目的:

为了研究CLL细胞中NOTCH1,DELTEX1,HES1和AIOLOS的基因和蛋白质表达.
分析与这些基因相关的BCL2和microRNAs (miRNAs) 的表达.
为了确定NOTCH通路的激活是否影响CLL细胞亡.

主要方法:

从外围血液淋巴细胞 (PBL) 和骨髓 (BM) 的初级CLL细胞中分析基因和蛋白质表达.
对NOTCH1,DELTEX1,HES1,AIOLOS,BCL2以及特定的miRNAs (miR-15a,miR-181,miR-146,miR-155) 的定量评估.
在体外实验中研究NOTCH通路激活对CLL细胞亡的影响.

主要成果:

在所有CLL样本中,BCL2和AIOLOS表达高,而PBL中的AIOLOS高于BM.
发现NOTCH1的激活在骨髓中更高.
在大多数样本中观察到miR-15a,miR-181,miR-146的表达减少,miR-155的表达增加.
在体外NOTCH通路的激活使CLL细胞对细胞亡敏感,而不管BCL2水平高.

结论:

在外周血液和骨髓中,CLL表现出明显的分子谱.
NOTCH通路的激活在CLL病变发生过程中起着重要作用,可能是治疗点.
调节NOTCH通路可以克服对CLL中BCL2调节的亡抵抗.

关键词:

艾奥洛斯 (AIOLOS) 是一个在BCL-2中.在 CLL CLL 中.没有任何东西.这种白血病是白血病.幸存率幸存率生存率

更多相关视频

HPLC-based Assay to Monitor Extracellular Nucleotide/Nucleoside Metabolism in Human Chronic Lymphocytic Leukemia Cells

HPLC-based Assay to Monitor Extracellular Nucleotide/Nucleoside Metabolism in Human Chronic Lymphocytic Leukemia Cells

Published on: July 20, 2016

From a 2DE-Gel Spot to Protein Function: Lesson Learned From HS1 in Chronic Lymphocytic Leukemia

From a 2DE-Gel Spot to Protein Function: Lesson Learned From HS1 in Chronic Lymphocytic Leukemia

Published on: October 19, 2014

相关概念视频

Interactions Between Signaling Pathways

Interactions Between Signaling Pathways

Signaling cascades usually lack linearity. Multiple pathways interact and regulate one another, allowing cells to integrate and respond to diverse environmental stimuli.
Convergence and divergence, and cross-talk between signaling pathways
Two distinct signaling pathways can converge on a single functional unit, which may either be a single protein or a complex of proteins. The response is either functionally distinct or synergistic between the two pathways but different from the response...

Diversity in Cell Signaling Responses

Diversity in Cell Signaling Responses

The physiological function of a cell and cellular communication are outcomes of a range of extrinsic signals, intracellular signaling pathways, and cellular responses. No two cell types express the same repertoire of signaling components. Receptors are highly selective for their cognate ligands, but once activated, they can alter multiple cellular processes such as DNA transcription, protein synthesis, and metabolic activity.
Graded and Abrupt Responses
Some signaling systems generate...

The JAK-STAT Signaling Pathway

The JAK-STAT Signaling Pathway

Several cytokine receptors have tightly bound Janus kinase or JAK proteins attached at their cytosolic tail. Small signaling molecules such as cytokines, growth hormones, or prolactins bind to the cytokine receptors and initiate their dimerization. The dimerization brings the cytosolic JAKs together that trans-phosphorylate and activates each other. The activated JAKs now phosphorylate cytosolic tails of the cytokine receptors, which serve as binding sites for adaptor proteins such as SH2...