人类染色体18号长跨基因非编码RNA:关注癌症
Pavel V Ershov1, Evgeniy O Yablokov1, Yuri V Mezentsev1
1Institute of Biomedical Chemistry, Moscow 119121, Russia.
Biomedicines
|March 28, 2024
概括
这项研究分析了人类染色体18上的65个长跨基因非编码RNA (lincRNAs),发现只有五个被证实与癌症相关. 许多lincRNAs表现出与癌症生物学相关的改变表达和相互作用.
科学领域:
- 基因组学就是基因组学.
- 癌症生物学 癌症生物学
- 分子瘤学分子瘤学
背景情况:
- 恶性瘤表现出显著的分子异质性.
- 非编码RNAs,特别是长跨基因非编码RNAs (lincRNAs),越来越多地被认为是它们在癌症发展中的作用.
- 人类染色体18号含有大量的lincRNAs,它们在癌症中起到很大程度上没有特征的作用.
研究的目的:
- 系统地分析人类染色体18上的65个lincRNA的基因组,转录组,表观组和相互作用组数据.
- 评估这些lincRNAs在癌症生物学中的泛癌相关性和潜在作用.
- 为了确定具有潜在预后价值的lincRNAs.
主要方法:
- 基因组,转录基因组,表观基因组和相互作用基因组数据集的数据挖掘.
- 文献综述和对现有的生物医学和分子生物学数据的分析.
- 功能丰富分析和转录密码签名的识别.
主要成果:
- 在65个分析的lincRNA中,只有5个被证实与癌症相关,另有11个显示潜在关联.
- 40%的染色体18 lincRNAs 呈现组织特异性表达,许多在癌症中受到基因组改变 (融合,放大,删除).
- 确定了lincRNA相互作用体,包括与microRNA和蛋白质的相互作用,并发现六个lincRNA编码小开放读取框架 (smORF) 蛋白质.
- 发现了7个转录组签名,包括2-7个具有潜在预后价值的lincRNA.
结论:
- 人类染色体18上的大部分长跨基因非编码RNA在癌症的背景下未得到充分研究.
- 基因组变化,差异表达和与关键分子参与者的相互作用表明染色体18 lincRNAs 在癌症中的作用.
- 对特定的lincRNA及其编码的smORF进行进一步的研究是有必要的,以阐明它们在致癌中的功能和作为治疗点的潜力.
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