选择的候选基因的基因型特异性表达 赋予抗力 叶子生的 (Secale cereale L.)
Rumana Azad1, Tomasz Krępski1, Mateusz Olechowski1
1Department of Plant Genetics, Breeding and Biotechnology, Institute of Biology, Warsaw University of Life Sciences, Nowoursynowska 166, 02-787 Warszawa, Poland.
Genes
|March 28, 2024
概括
叶的抗生素包括四个关键基因:DXS,Glu,GT和PR-1. 耐药和易受性麦系之间的基因表达模式不同,为植物对Puccinia recondita的免疫提供了洞察力.
科学领域:
- 植物病理学 植物病理学
- 分子生物学分子生物学
- 遗传学 是一个遗传学.
背景情况:
- 叶子生 (LR),是由Puccinia recondita f. sp.引起的 (Prs),对麦生产构成重大威胁.
- 对于麦对LR的免疫反应的遗传基础尚不清楚.
研究的目的:
- 为了研究Prs接种后的杂血统中四个特定基因 (DXS,Glu,GT,PR-1) 的表达模式.
- 为了将基因表达与观察到的叶子抗性水平相关联.
主要方法:
- 使用定量实时PCR (RT-qPCR) 来分析基因表达.
- 微观和宏观评估评估了叶子生感染水平.
- 对DXS (1-deoxy-D-xylulose 5-phosphate synthase),Glu (β-1,3-glucanase),GT (UDP-glycosyltransferase) 和PR-1 (致病相关蛋白1) 的表达在治疗后20小时和36小时进行测量.
主要成果:
- 所有四个基因都在所有12个麦杂交系和两个时间点中显示出对Prs的反应上调表达.
- 八条线表现出对LR的敏感性,而四条显示出高耐药性.
- 抗性线通常在20hpt时表现出更高的基因表达折叠变化,而在36hpt时表现出更高的基因表达折叠变化,而敏感线则显示出相反的趋势.
结论:
- 基因DXS,Glu,GT和PR-1对麦的抗叶病原体防御机制具有潜在的关键作用.
- 不同的基因表达动态与麦中不同程度的耐药性相关.
- 这项研究提供了关于麦对叶子生的免疫反应的宝贵遗传见解.
关键词:
普奇尼亚·雷康迪塔 (Puccinia recondita) f. sp. 的一种植物. 这里是Secalis secalis 的位置.实时PCR定量的实时PCR.抗性基因是抵抗性的基因.幼苗阶段的耐药性 幼苗阶段的耐药性冬季的麦 冬季的麦更多相关视频
08:36Development of Targeting Induced Local Lesions IN Genomes TILLING Populations in Small Grain Crops by Ethyl Methanesulfonate Mutagenesis
Published on: July 16, 2019
11.6K
10:19Screening and Identification of RNA Silencing Suppressors from Secreted Effectors of Plant Pathogens
Published on: February 3, 2020
6.4K
相关概念视频
01:21Frequency-dependent Selection
When the fitness of a trait is influenced by how common it is (i.e., its frequency) relative to different traits within a population, this is referred to as frequency-dependent selection. Frequency-dependent selection may occur between species or within a single species. This type of selection can either be positive—with more common phenotypes having higher fitness—or negative, with rarer phenotypes conferring increased fitness.Positive Frequency-Dependent SelectionIn positive...
02:27Trihybrid Crosses
Trihybrid Crosses
Some of Mendel’s crosses examined three pairs of contrasting characteristics. Such a cross is called a trihybrid cross. A trihybrid cross is a combination of three individual monohybrid crosses. For example, plant height (tall vs. short), seed shape (round vs. wrinkled), and seed color (yellow vs. green).
The F1 generation plants of a trihybrid cross are heterozygous for all three traits and produce eight gametes. Upon self-fertilization, these gametes have an equal chance to...
Some of Mendel’s crosses examined three pairs of contrasting characteristics. Such a cross is called a trihybrid cross. A trihybrid cross is a combination of three individual monohybrid crosses. For example, plant height (tall vs. short), seed shape (round vs. wrinkled), and seed color (yellow vs. green).
The F1 generation plants of a trihybrid cross are heterozygous for all three traits and produce eight gametes. Upon self-fertilization, these gametes have an equal chance to...