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一个可扩展和坚固的叶绿体基因型化解决方案:在玉米叶绿体基因组中开发和应用SNP和InDel标记物

Rui Wang¹, Yang Yang¹, Hongli Tian¹

¹Maize Research Institute, Beijing Key Laboratory of Maize DNA Fingerprinting and Molecular Breeding, Key Laboratory of Crop DNA Fingerprinting Innovation and Utilization (Co-construction by Ministry and Province), Beijing Academy of Agricultural and Forest Sciences (BAAFS), Beijing 100097, China.

|March 28, 2024

概括

这项研究开发了使用SNP和InDel标记物用于玉米的种类质塑料面板 (VCP). 这些标记物有效地识别了叶绿体群,并有助于了解母亲的遗传和玉米的育种.

科学领域:

植物遗传学和育种.
分子生物学分子生物学
农业科学农业科学

背景情况:

了解玉米的遗传多样性对于作物改进和进化研究至关重要.
核标记在表征细胞质特征方面存在局限性,例如男性不育和杂交起源.
叶绿体DNA标记物为遗传分析提供了一个补充的方法.

研究的目的:

开发和验证一个高通量标记系统,用于玉米质细胞的基因定型.
评估这些标记物在识别不同玉米品种群的有用性.
证明质体标记物在特征母性遗传和人工选择中的应用.

主要方法:

下一代测序176个玉米样本以确定遗传变异.
开发49个SNP和InDel标记物,将其转化为种类塑板 (VCP).
使用竞争性等位基特定PCR (KASP) 进行高通量标记物检测.

主要成果:

确定了89个单核酸多态 (SNPs) 和11个插入/删除多态 (InDels).
成功开发和验证了49个SNP/InDel标记器作为VCP.
VCP标记器有效地区分了玉米的叶绿体群,并确定了相互交叉和细胞质男性不育混合体.

结论:

该VCP提供了一个强大而高效的工具,用于玉米的叶绿体鉴定和母系遗传研究.
这些标记物对植物育种,植物遗传学分析和了解玉米化有价值.
一个小的VCP标记子集足以阐明精英玉米系中的人工选择.

关键词:

在InDel,我们可以使用它.在SNP中,SNP是SNP.类塑料是一种塑料.基因型定制是基因型定制.高吞吐量,具有高吞吐量.玉米玉米玉米是一种

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