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在大规模死亡事件后,在环境温度下长期保存组织,以基于DNA的受害者鉴定为基础

Xavier Liang Shun Chan¹, Shumei Michelle Lai¹, Danial Asyraaf Bin Hamdan¹

¹DNA Profiling Laboratory, Biology Division, Health Sciences Authority, 11 Outram Road, Singapore 169078, Singapore.

|March 28, 2024

概括

在大规模死亡事件中,非盐和酒精有效地保护组织以进行DNA识别,即使在环境温度下也是如此. 与其他方法相比,盐在24个月内显示出优越的DNA完整性保护.

科学领域:

法医科学法医科学法医科学
分子生物学分子生物学
生物化学生物化学

背景情况:

在大规模死亡事件 (MFI) 中,有效的组织保存对于基于DNA的受害者识别至关重要.
在长时间停电期间,如武装冲突中,冷等传统方法可能是不切实际的.
对于在环境温度下长期储存,需要使用替代的,低成本的保存方法.

研究的目的:

为了比较使用盐和酒精与化学保存和冷相对应的环境温度组织保存的长期有效性.
为了评估下游短串重复聚合酶链反应 (STR-PCR) 分析的DNA完整性和片大小.

主要方法:

牛肌肉组织使用非盐,40%的酒精饮料,AllprotectTM组织反应剂,并在-20°C冷保存.
在24个月的时间内,在6个时间点分析了样本.
通过凝电泳检测评估了DNA完整性,并确定了STR-PCR片的大小.

主要成果:

所有四种保存方法都保持了组织活力,并在24个月内产生了STR-PCR安普利康> 200bp.
非盐显示出 DNA 完整性的优越保存,在凝电泳术上可见高分子量 DNA.
其他方法导致了低分子量DNA涂抹,表明降解更大.

结论:

非盐提供了一种简单,低成本和有效的方法,用于在环境温度下长期保存组织,以识别MFI受害者.
这种方法在资源有限的环境中或在电力不稳定的紧急情况下特别有价值.
进一步的研究可以完善用于法医应用的环境温度保护协议.

关键词:

酒精酒精酒精酒精酒精酒精酒精酒精化学防腐剂是一种化学防腐剂.结结结结是一种盐盐盐盐盐盐盐盐盐

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