分散固相提取和超高性能液态染色学-并联质谱-一种快速而准确的方法,用于检测水生产品中的10种宏类残留物
Jinyu Chen1,2, Guangming Mei2, Xiaojun Zhang2
1College of Food and Pharmaceutical Science, Zhejiang Ocean University, Zhoushan 316022, China.
Foods (Basel, Switzerland)
|March 28, 2024
概括
一种新方法使用UPLC-MS/MS.准确检测海鲜中的十种宏类 (MAL) 抗生素残留物. 这种经过验证的技术通过量化这些常见的兽药残留物来确保食品安全.
科学领域:
- 分析化学 分析化学
- 食品安全 食品安全
- 环境科学 环境科学
背景情况:
- 宏类 (MAL) 抗生素在畜牧业中被广泛使用,导致水产品中的潜在残留物.
- 准确检测MAL残留物对于确保消费者安全和海鲜行业的监管合规至关重要.
- 现有的方法可能缺乏全面MAL残留分析所需的灵敏度,速度或范围.
研究的目的:
- 开发和验证一种敏感且可靠的方法,用于量化水产品中的十大宏化物 (MAL) 抗生素残留物.
- 建立强大的分析参数,包括线性,检测极限,量化极限,回收和精度.
- 为定期监测海鲜中MAL污染提供一个工具.
主要方法:
- 固体相提取 (SPE) 与中性吸附用于样品清洁.
- 超高性能液态染色学-并联质谱学 (UPLC-MS/MS) 用于分离和检测.
- 精度UPLC BEH C18柱和乙尼-0.1%酸移动相;正态ESI和MRM扫描.
主要成果:
- 在1.0-100μg/L的度范围内,UPLC-MS/MS方法对10个MALs表现出极好的线性 (R2>0.99).
- 检测极限 (LOD) 在0.25-0.50μg/kg之间,量化极限 (LOQ) 在0.5-1.0μg/kg之间.
- 实现了高恢复率 (83.1-116.6%) 和良好的精度 (一天内3.7%,一天内13.8%).
结论:
- 开发的UPLC-MS/MS方法简单,快速,准确,可重复,用于十种宏类残留物的定性和定量分析.
- 这种经过验证的方法符合有效监测水产品中MAL抗生素污染的要求.
- 这些发现有助于加强海鲜食品安全协议.
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