Jove
Visualize
联系我们
JoVE
x logofacebook logolinkedin logoyoutube logo
关于 JoVE
概览领导团队博客JoVE 帮助中心
作者
出版流程编辑委员会范围与政策同行评审常见问题投稿
图书馆员
用户评价订阅访问资源图书馆顾问委员会常见问题
研究
JoVE JournalMethods CollectionsJoVE Encyclopedia of Experiments存档
教育
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab Manual教师资源中心教师网站
使用条款与条件
隐私政策
政策

相关概念视频

MicroRNAs01:22

MicroRNAs

3.0K
MicroRNA (miRNA) are short, regulatory RNA transcribed from introns (non-coding regions of a gene) or intergenic regions (stretches of DNA present between genes). Several processing steps are required to form biologically active, mature miRNA. The initial transcript, called primary miRNA (pri-mRNA), base-pairs with itself, forming a stem-loop structure. Within the nucleus, an endonuclease enzyme, called Drosha, shortens the stem-loop structure into hairpin-shaped pre-miRNA. After the pre-miRNA...
3.0K
Ribosome Profiling02:24

Ribosome Profiling

3.5K
Ribosome profiling or ribo-sequencing is a deep sequencing technique that produces a snapshot of active translation in a cell. It selectively sequences the mRNAs protected by ribosomes to get an insight into a cell’s translation landscape at any given point in time.
Applications of ribosome profiling
Ribosome profiling has many applications, including in vivo monitoring of translation inside a particular organ or tissue type and quantifying new protein synthesis levels.
The technique...
3.5K

您也可能阅读

相关文章

通过共同作者、期刊和引用图与本文相关的文章。

排序
Same author

Novel Molecular Insights into the Anti-Inflammatory and Antifibrotic Effects of Dexamethasone on Human Ligamentum Flavum-Derived Cells.

International journal of molecular sciences·2026
Same author

Bioactivity of Aromatic Plant Hydrolates and Application for Pork Marination.

Antioxidants (Basel, Switzerland)·2025
Same author

SWATH-MS proteomics data on differentially abundant proteins between normal and dark-cutting beef.

Data in brief·2025
Same author

Redox Status in Patients Suffering from Multiple Chemical Sensitivity (MCS): A Pilot Study.

Journal of clinical medicine·2025
Same author

The Hormetic Adaptative Capacity and Resilience to Oxidative Stress Is Strengthened by Exposome Enrichment with Air Cold Atmospheric Plasma: A Metabolome Targeted Follow-Up Approach.

Biomedicines·2025
Same author

Multicomponent nucleic acid enzymes as signal amplification strategy for the detection of microRNA based on fluorescence resonance energy transfer.

Mikrochimica acta·2025

相关实验视频

Updated: Jun 29, 2025

Lung microRNA Profiling Across the Estrous Cycle in Ozone-exposed Mice
07:07

Lung microRNA Profiling Across the Estrous Cycle in Ozone-exposed Mice

Published on: January 7, 2019

6.2K

在深色切割牛肉中探索miRNAs配置文件

Laura González-Blanco1,2, Luis J Royo1,3, Yolanda Diñeiro1,2

  • 1Área de Sistemas de Producción Animal, Servicio Regional de Investigación y Desarrollo Agroalimentario (SERIDA), Ctra. AS-267, PK 19, 33300 Villaviciosa, Spain.

Foods (Basel, Switzerland)
|March 28, 2024
PubMed
概括

压力因素会耗尽肌肉的糖原,导致黑色,坚硬和干燥的 (DFD) 牛肉. 死后24小时肌肉中的微RNA (miRNA) 差异表达表明早期牛肉质量缺陷,与细胞应激反应有关.

关键词:
在DFD牛肉.这就是米RNA.肉质 肉质 肉质 肉质氧化应激是一种氧化应激.这是骨肌肉的骨架肌肉.

更多相关视频

Profiling of Estrogen-regulated MicroRNAs in Breast Cancer Cells
16:24

Profiling of Estrogen-regulated MicroRNAs in Breast Cancer Cells

Published on: February 21, 2014

20.2K
A Complete Pipeline for Isolating and Sequencing MicroRNAs, and Analyzing Them Using Open Source Tools
00:09

A Complete Pipeline for Isolating and Sequencing MicroRNAs, and Analyzing Them Using Open Source Tools

Published on: August 21, 2019

7.3K

相关实验视频

Last Updated: Jun 29, 2025

Lung microRNA Profiling Across the Estrous Cycle in Ozone-exposed Mice
07:07

Lung microRNA Profiling Across the Estrous Cycle in Ozone-exposed Mice

Published on: January 7, 2019

6.2K
Profiling of Estrogen-regulated MicroRNAs in Breast Cancer Cells
16:24

Profiling of Estrogen-regulated MicroRNAs in Breast Cancer Cells

Published on: February 21, 2014

20.2K
A Complete Pipeline for Isolating and Sequencing MicroRNAs, and Analyzing Them Using Open Source Tools
00:09

A Complete Pipeline for Isolating and Sequencing MicroRNAs, and Analyzing Them Using Open Source Tools

Published on: August 21, 2019

7.3K

科学领域:

  • 动物科学动物科学
  • 分子生物学分子生物学
  • 食品科学 食品科学 食品科学

背景情况:

  • 动物生产压力因素可以耗尽肌肉糖原储存,导致死亡后pH值升高 (pH24).
  • 这种代谢变化导致肉质缺陷,特别是深色,坚硬和干燥的 (DFD) 牛肉.
  • 小型非编码RNA或微RNA (miRNA) 参与调节受压力影响的细胞过程.

研究的目的:

  • 调查死后24小时长胸筋和肌肉中的差异性miRNA表达是否可以预测牛肉质量缺陷.
  • 为了确定与深色,坚硬和干燥 (DFD) 牛肉的发展相关的特定miRNAs.

主要方法:

  • 在肌肉组织上进行了总RNA提取和全面的miRNAome测序.
  • 在DFD和对照牛肉样本之间进行了微分miRNA表达分析.
  • 定量RT-PCR验证了特定的miRNA表达,而西式涂抹分析了标蛋白水平.

主要成果:

  • 与对照牛肉相比,DFD牛肉中的12个miRNAs被显著上调 (p < 0.001).
  • 上调的miRNAs主要参与细胞对氧化应激和亡的反应.
  • DFD牛肉显示高70kDa的热冲击蛋白和低的Caspase-9表达,表明亡下调.

结论:

  • 死亡后24小时的微RNA差异丰度是牛肉质量缺陷的早期指标.
  • 在死后早期,miRNAs在调节肌肉细胞应激反应方面发挥着至关重要的作用.
  • 改变的miRNA表达会影响肌肉转化为肉类,导致不良的肉质特征,如DFD牛肉.