血小板激活因子受体 (PAFR) 调节视网膜原生细胞/干细胞在上皮细胞中的特征
Barbara Dalmaso1, Ildefonso Alves da Silva-Junior2, Sonia Jancar2
1Department of Cell and Developmental Biology, Biomedical Sciences Institute, University of Sao Paulo, Sao Paulo 05508-000, Brazil.
International journal of molecular sciences
|March 28, 2024
概括
血小板激活因子受体 (PAFR) 信号影响视网膜干细胞特征. 阻断PAFR增强了祖先标记物,这表明它在视网膜发育和修复中的作用.
科学领域:
- 眼科医生 眼科 眼科
- 神经科学是一个神经科学.
- 干细胞生物学 干细胞生物学
背景情况:
- 哺乳动物视网膜缺乏低脊椎动物中发现的内在再生能力.
- 哺乳动物的上皮 (CE) 细胞可以被重新编程成视网膜干细胞,但其重新编程潜力有限.
- 血小板激活因子 (PAF) 信号传递对于干细胞的增殖和分化至关重要,在视网膜前体发育中具有已知的作用.
研究的目的:
- 为了研究血小板激活因子受体 (PAFR) 在上皮质 (CE) 衍生的干细胞中信号传递的作用.
- 探索PAFR信号如何影响干细胞特征,神经圈形成和视网膜中的祖先标记物表达.
主要方法:
- 分析PAF受体和CE衍生的祖先/干细胞中的相关酶表达.
- 使用特定抗剂抑制PAFR活性.
- 在PAFR阻塞后评估原生细胞标记物和神经圈形成.
主要成果:
- 发现PAFR和相关酶在CE衍生的视网膜前体/干细胞中被下调.
- 用抗体阻断PAFR活性导致关键原生细胞标记物的表达增加.
- 表明PAFR信号动态影响干细胞特性和CE细胞中神经圈的形成.
结论:
- PAFR信号传递在调节来自皮上皮的视网膜干细胞的特征方面发挥着重要作用.
- 调节PAFR活性为增强视网膜原生细胞功能提供了潜在的治疗策略,有助于视网膜组织的发育和维护.
相关概念视频
01:01Regulation of Hematopoietic Stem Cells
3.2K
All blood and immune cells are produced from the multipotent hematopoietic stem cells (HSCs) by the process of hematopoiesis. However, they all have a limited life span. In addition, many are depleted in immune surveillance or combatting an injury or infection. This makes blood one of the most regenerative tissues. Hematopoiesis helps replenish these blood and immune cells, restoring the body's normal functioning. However, overproduction of blood and immune cells can make them cancerous or...
3.2K
01:24Regulation of Angiogenesis and Blood Supply
2.6K
Rapidly dividing tumors, embryos, and wounded tissues require more oxygen than usual, lowering the oxygen concentration in the blood. At low oxygen or hypoxic conditions, an oxygen-sensitive transcription factor called the hypoxia-inducible factor 1 or HIF1 is activated. HIF1 is a dimeric protein of alpha (ɑ) and beta (β) subunits. Under optimal oxygen conditions, HIF1β is present in the nucleus while HIF1ɑ remains in the cytosol. HIF1ɑ is hydroxylated by prolyl...
2.6K
01:18Structure and Function of Platelets
1.1K
The cell fragments known as platelets are disc-shaped, with an average diameter of about 3 μm and a thickness of roughly 1 μm. They play a crucial role in the body's vascular clotting system, which also involves plasma proteins, blood cells, and blood vessel tissues.
Platelets are continually replenished, circulating in the bloodstream for 9-12 days before being removed by phagocytes, primarily in the spleen. A microliter of circulating blood contains between 150,000 and 450,000...
Platelets are continually replenished, circulating in the bloodstream for 9-12 days before being removed by phagocytes, primarily in the spleen. A microliter of circulating blood contains between 150,000 and 450,000...
1.1K