在Xanthoceras sorbifolium中受精后,斯特里戈拉克顿可能会调节卵子的发育
Qingyuan Zhou1, Linyi Zhou2, Qing Cai1
1Institute of Botany, Chinese Academy of Sciences, Xiangshan, Beijing 100093, China.
International journal of molecular sciences
|March 28, 2024
概括
斯特里戈拉克顿调节了Xanthoceras sorbifolium中的种子发育. 它们与糖的相互作用影响卵子流产,为改善种子产量提供了见解.
科学领域:
- 植物激素信号传递器
- 种子的发育和繁殖.
- 植物生理学 植物生理学
背景情况:
- 斯特里戈拉克顿 (SLs) 是众所周知的调节生长和发育的植物激素.
- 它们在早期种子发育和卵子流产中的作用仍然在很大程度上未被探索.
- 桑托塞拉斯皮叶 (Xanthoceras sorbifolium) 为研究这些过程提供了一个模型.
研究的目的:
- 为了调查strigolactones在Xanthoceras sorbifolium受精卵中的存在和作用.
- 为了阐明strigolactones,糖代谢和卵子流产之间的关系.
- 确定提高种子产量的潜在目标.
主要方法:
- 在不同的酸盐条件下,在发育中的卵子中量化strigolactones.
- 斯特里戈拉克类比 (GR24) 和抑制剂 (TIS108) 的应用.
- 对SL相关基因进行全基因组识别和RNA序列分析.
- 对卵子发育和流产的转录组分析.
- 糖的代谢物分析 (六合糖,糖糖).
- 病毒诱导的XsD14*基因沉默 (VIGS).
主要成果:
- 在受精卵子和骨中检测到微小量的5-deoxystrigol和strigol,随着酸盐的添加而减少.
- SL类似和抑制剂治疗影响了种子发育和果.
- 37个SL相关基因在正常卵子和堕胎卵子之间显示出差异性表达.
- 正常发育的卵子显示出高赫索积和活跃的逆转酶基因表达.
- 堕胎的卵子具有较低的赫索索/糖分比率和高的斯特里戈拉克含量.
- *XsD14* VIGS增加了黑色素含量,促进了内的发育,并改善了果.
结论:
- 斯特里戈拉克顿存在于早期发育的种子中,并影响卵子发育.
- 糖和strigolactone信号之间的交叉声调节了卵子流产.
- 了解这种交叉可以指导提高Xanthoceras种子产量的策略.
相关概念视频
02:39The Angiosperm Life Cycle
65.1K
Plants have a life cycle split between two multicellular stages: a haploid stage—with cells containing one set of chromosomes—and a diploid stage—with cells containing two sets of chromosomes. The haploid stage is the gamete-producing gametophyte, and the diploid stage is the spore-producing sporophyte.
65.1K
02:04Regulation of Transpiration by Stomata
28.2K
During photosynthesis, plants acquire the necessary carbon dioxide and release the produced oxygen back into the atmosphere. Openings in the epidermis of plant leaves is the site of this exchange of gasses. A single opening is called a stoma—derived from the Greek word for “mouth.” Stomata open and close in response to a variety of environmental cues.
28.2K
02:33Seed Structure and Early Development of the Sporophyte
28.2K
Seed structures are composed of a protective seed coat surrounding a plant embryo, and a food store for the developing embryo. The embryo contains the precursor tissues for leaves, stem, and roots. The endosperm and cotyledons—seed leaves—act as the food reserves for the growing embryo.
28.2K
01:27Feedback Regulation of Calcium Concentration
3.4K
Calcium is an essential signaling molecule required for various cellular functions. Calcium pumps and ion channels on cell and organellar membranes, such as those on the endoplasmic reticulum (ER), regulate calcium concentrations inside the cell. They remain closed, keeping the cytosolic calcium levels low at a resting state.
Various transmembrane receptors, such as G protein-coupled receptors (GPCRs), elicit a response to extracellular signals by increasing cytosolic calcium. Activated GPCRs...
Various transmembrane receptors, such as G protein-coupled receptors (GPCRs), elicit a response to extracellular signals by increasing cytosolic calcium. Activated GPCRs...
3.4K
01:27Ovarian Cycle
1.2K
The menstrual cycle includes a critical component known as the ovarian cycle, which undergoes two main phases each month—the follicular phase and the luteal phase. The follicular phase is variable and averaging around 14 days. Ovulation, triggered by a surge in luteinizing hormone (LH), marks the transition between the two phases. The second phase, the luteal phase, is relatively consistent, lasting approximately 14 days, and is marked by the activity of the corpus luteum. While a cycle...
1.2K
01:20Folliculogenesis
744
Folliculogenesis is the development of ovarian follicles, the specialized structures within the ovarian cortex where oogenesis, or egg development, occurs. This process is essential for female reproductive health and begins during fetal development when primordial follicles are formed. Each primordial follicle comprises a primary oocyte in the center, surrounded by a single layer of squamous pre-granulosa cells. These follicles remain dormant in late prophase I of meiosis until triggered by...
744