相关实验视频

Updated: Jun 7, 2026

RNA Isolation from Embryonic Zebrafish and cDNA Synthesis for Gene Expression Analysis
15:21

RNA Isolation from Embryonic Zebrafish and cDNA Synthesis for Gene Expression Analysis

Published on: August 7, 2009

38.2K

在斑马鱼的三个关键机体生成阶段对长非编码RNA的系统识别

Chune Zhou1, Mengting Li1, Yaoyi Sun1

  • 1Henan International Joint Laboratory of Aquatic Ecotoxicology and Health Protection, College of Life Sciences, Henan Normal University, Xinxiang 453007, China.

International journal of molecular sciences
|March 28, 2024
PubMed
概括

这项研究确定了长非编码RNA gas5 (lncRNA gas5) 对于斑马鱼胚胎肌肉发育至关重要. 淘汰 lncRNA gas5 干扰了骨肌肉的形成,强调了它在器官生成中的作用.

关键词:
在cRNA气体5中长长的非编码RNAs.肌肉的发展 肌肉的发展 肌肉的发展系统的识别和系统的识别.机体发生的三个关键阶段.斑马鱼是一种斑马鱼.

更多相关视频

Large-scale Zebrafish Embryonic Heart Dissection for Transcriptional Analysis10:00

Large-scale Zebrafish Embryonic Heart Dissection for Transcriptional Analysis

Published on: January 12, 2015

14.5K
Sample Preparation and Analysis of RNASeq-based Gene Expression Data from Zebrafish11:42

Sample Preparation and Analysis of RNASeq-based Gene Expression Data from Zebrafish

Published on: October 27, 2017

10.9K

相关概念视频

RNA Splicing01:32

RNA Splicing

Splicing is the process by which eukaryotic RNA is edited before its translation into protein. The RNA strand transcribed from eukaryotic DNA is called the primary transcript. The primary transcripts that become mRNAs are called precursor messenger RNAs (pre-mRNAs). Eukaryotic pre-mRNA contains alternating sequences of exons and introns. Exons are nucleotide sequences that code for proteins, whereas introns are the non-coding regions. In RNA splicing, introns are removed and exons are bonded...
RNA Splicing01:32

RNA Splicing

Splicing is the process by which eukaryotic RNA is edited before its translation into protein. The RNA strand transcribed from eukaryotic DNA is called the primary transcript. The primary transcripts that become mRNAs are called precursor messenger RNAs (pre-mRNAs). Eukaryotic pre-mRNA contains alternating sequences of exons and introns. Exons are nucleotide sequences that code for proteins, whereas introns are the non-coding regions. In RNA splicing, introns are removed and exons are bonded...
Nonsense-mediated mRNA Decay02:27

Nonsense-mediated mRNA Decay

The Upf proteins that carry out nonsense-mediated decay (NMD) are found in all eukaryotic organisms, including humans. Each protein has an individual role, but they need to work in collaboration. Upf1 is an ATP-dependent RNA helicase that unwinds the RNA helix. Because Upf1 can unwind any RNA, Upf2 and Upf3 are required to help Upf1 discriminate between nonsense and normal mRNAs.
Usually, Upf3 binds to an Exon Junction Complex (EJC) at mRNA splice sites. If a ribosome fully translates the mRNA,...
Leaky Scanning02:28

Leaky Scanning

During most eukaryotic translation processes, the small 40S ribosome subunit scans an mRNA from its 5' end until it encounters the first start AUG codon. The large 60S ribosomal subunit then joins the smaller one to initiate protein synthesis. The location of the translation initiation is largely determined by the nucleotides near the start codon as there may be multiple translation initiation sites present on the mRNA.  Marilyn Kozak discovered that the sequence RCCAUGG (where R stands for...
RNA-seq03:21

RNA-seq

RNA sequencing, or RNA-Seq, is a high-throughput sequencing technology used to study the transcriptome of a cell. Transcriptomics helps to interpret the functional elements of a genome and identify the molecular constituents of an organism. Additionally, it also helps in understanding the development of an organism and the occurrence of diseases. 
Before the discovery of RNA-seq, microarray-based methods and Sanger sequencing were used for transcriptome analysis. However, while microarray-based...
Ribosome Profiling02:24

Ribosome Profiling

Ribosome profiling or ribo-sequencing is a deep sequencing technique that produces a snapshot of active translation in a cell. It selectively sequences the mRNAs protected by ribosomes to get an insight into a cell’s translation landscape at any given point in time.
Applications of ribosome profiling
Ribosome profiling has many applications, including in vivo monitoring of translation inside a particular organ or tissue type and quantifying new protein synthesis levels.
The technique helps...