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在百合花中的球泡形成期间,假定糖载体基因家族的识别和表达分析

Ziyang Huang¹, Cong Gao², Yunchen Xu²

¹Laboratory of Flower Bulbs, Department of Landscape Architecture, Zhejiang Sci-Tech University, Hangzhou 310018, China.

International journal of molecular sciences

|March 28, 2024

概括

这项研究确定了百合中的许多糖载体,揭示了LohSTP8,LohSTP12和LflERD6.3等特定的载体,这些载体对百合球泡的形成和发育至关重要.

科学领域:

植物生物学植物生物学
分子生物学分子生物学
基因组学就是基因组学.

背景情况:

糖运输体对于植物生长,发育和应激反应至关重要.
糖代谢对于百合花的球泡形成至关重要 (Lilium spp. ),装饰型球形花.
了解糖载体的作用对于分析百合球泡的发育至关重要.

研究的目的:

在两个百合品种中识别和描述糖载体:东方杂交"Sorbonne"和"Lilium" × "formolongi".
分析花球形成期间糖载体的表达模式.
确定潜在的关键糖运输商参与百合球的启动和发展.

主要方法:

对转录组数据进行生物信息学分析,以识别糖载体.
植物遗传学分析来分类单糖运输体 (MST),糖糖运输体 (SUT) 和糖最终将成为出口运输体 (SWEET).
保存的动机和域分析.
克隆和LohINT1的亚细胞定位.
在不同发育阶段和治疗方法下进行基因表达分析 (转录组和qRT-PCR).

主要成果:

在"索尔本"确定了69个糖运输商,在"L"确定了41个糖运输商.
遗传学分析揭示了MST,SUT和SWEET的不同的子家族/组.
预测这些传送器家族的保存动机和域名.
证实LohINT1位于细胞膜中.
在两种百合花品种中,在球球开始和地下球球生长期间,发现特定的糖载体受到上调.

结论:

该研究提供了一个全面的识别和表征的糖运输器在百合.
特定的糖运输体,包括LohSTP8,LohSTP12和LflERD6.3,作为球形成的关键调节体,具有强烈的影响.
需要进行进一步的功能验证,以确认这些已识别的糖载体在百合球泡发育中的确切作用.

关键词:

柳叶花柳叶花柳叶花柳叶花柳叶花柳叶花球形发展的发展.球球启动启动表达式分析,表达式分析.糖运输公司糖运输公司

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