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血液和精液检测的评估和DNA采集从抽样在五种不同的布料材料上沉积后长达三个月的抽样

Francisco Medina-Paz¹, Brandon Kuba¹, Emily Kryvorutsky¹

¹Department of Chemistry and Environmental Sciences, New Jersey Institute of Technology, Newark, NJ 07102, USA.

International journal of molecular sciences

|March 28, 2024

概括

法医科学家可以使用免疫染色体测试和STR分析可靠地识别各种织物上的血液和精液,直到沉积后90天,这有助于刑事调查.

科学领域:

法医科学法医科学法医科学
生物化学生物化学

背景情况:

体液识别在刑事调查中至关重要,血液和精液是最常见的.
基于抗原-抗体反应的血清分析,可快速和精确地检测这些体液.

研究的目的:

调查不同织物和沉积后的时间对血液和精液识别的影响.
为了评估DNA隔离和STR分析成功从各种基板上老化的体液样本.

主要方法:

血液和精液被沉积在牛仔,棉花和皮革织物上.
样品是在沉积后的1,30和90天收集的.
鉴定使用了免疫染色体测试 (SERATEC® HemDirect,PSA半量,SERATEC® BLOOD CS,SEMEN CS) 和DNA STR分析.

主要成果:

实验室和模拟犯罪现场测试都在检测所有织物上的血液和精液污点方面表现相似.
在任何面料的时间点之间没有观察到带强度的显著差异.
从血液和精液样本中获得完整的STR概况,直到沉积后三个月.
随着时间的推移,STR概况的质量呈现出变化,除了黑棉上的精液.

结论:

免疫染色学测试和STR分析是有效的识别血液和精液在普通织物上长达90天.
织物类型和沉积后的时间对初始识别的影响最小,但可能会影响STR资料质量.
进一步的研究应该探索额外的基板和环境条件.

关键词:

在STR的配置文件.血液血液血液血液织物织物织物织物织物织物侧流免疫染色学测试侧流免疫染色学测试精子精子精子精子时间时间时间时间时间.

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