相关实验视频

Updated: Jun 29, 2025

Highly Sensitive and Quantitative Detection of Proteins and Their Isoforms by Capillary Isoelectric Focusing Method

Highly Sensitive and Quantitative Detection of Proteins and Their Isoforms by Capillary Isoelectric Focusing Method

Published on: September 19, 2018

一种免疫FET与免疫磁性预度技术相结合,用于对高频生物标志物的敏感EIS检测

Hamdi Ben Halima¹, Nadia Zine¹, Imad Abrao Nemeir^1,2

¹Institut de Sciences Analytiques (ISA)-UMR 5280, Université Claude Bernard Lyon 1, 5 Rue de la Doua, 69100 Lyon, France.

|March 28, 2024

概括

这项研究引入了一种新型的三明治试验,用于检测心力衰竭生物标志物,瘤亡因子-α (TNF-α) 和互白素-10 (IL-10),使用磁纳米粒子和ISFET来提高灵敏度.

科学领域:

生物医学工程生物医学工程
纳米技术纳米技术
免疫感知是一种免疫感知.

背景情况:

像TNF-α和IL-10这样的心力衰竭 (HF) 生物标志物对于诊断至关重要.
现有的检测方法往往缺乏灵敏度,需要复杂的程序.
需要快速,灵敏和可靠的HF治疗诊断工具.

研究的目的:

开发一种新型的三明治免疫试验,用于同时检测TNF-α和IL-10.
使用磁纳米粒子 (MNP) 进行生物标志物预度和ISFET进行敏感检测.
为了实现低检测极限和高灵敏度的高频生物标志物量化.

主要方法:

使用BS3交叉链接器对MNP与单克隆抗体 (mAbs) 的生物功能化.
将多克隆抗体 (pAbs) 固定在ISFET上用于TNF-α和IL-10捕获.
电化学阻抗光谱 (EIS) 用于监测生物识别事件.
在人工唾液样本中量化标准添加方法.

主要成果:

开发的ImmunoFET显示,目标分析物的低检测极限为0.03ppg/ml.
与之前报告的电化学免疫传感器相比,对高频生物标志物的灵敏度更高.
精确量化人工唾液样本中的生物标志物,使用标准添加法.

结论:

拟议的三明治测定策略提供了一种敏感且高效的方法来检测HF生物标志物.
许多MNP和ISFET的组合为开发先进的免疫传感器提供了一个有前途的平台.
这种方法有可能用于早期诊断和监测心力衰竭.

关键词:

电化学阻抗光谱学电化学阻抗光谱学心脏衰竭是因为心脏衰竭.介质蛋白-10 介质蛋白-10 的作用.唾液分析唾液分析瘤死亡因子-α

更多相关视频

Development and Validation of an Ultrasensitive Single Molecule Array Digital Enzyme-linked Immunosorbent Assay for Human Interferon-α

Development and Validation of an Ultrasensitive Single Molecule Array Digital Enzyme-linked Immunosorbent Assay for Human Interferon-α

Published on: June 14, 2018

Detection of Exosomal Biomarker by Electric Field-induced Release and Measurement EFIRM

Detection of Exosomal Biomarker by Electric Field-induced Release and Measurement EFIRM

Published on: January 23, 2015

相关概念视频

Immunoprecipitation

Immunoprecipitation

Immunoprecipitation, or IP, is a widely used technique that employs protein-antibody interactions to isolate proteins or protein complexes in their native state for studying protein-protein interactions, quaternary structures, or supramolecular complexes. Various modifications of the technique, including chromatin IP, cross-linking IP, and fluorescence IP, are commonly used.
Chromatin Immunoprecipitation
Chromatin immunoprecipitation, also known as ChIP, is used to study protein-DNA or...

Enzyme-Linked Immunosorbent Assay

Enzyme-Linked Immunosorbent Assay

In 1971, Peter Perlman and Eva Engvall developed an Enzyme-linked immunosorbent assay (ELISA or EIA). ELISA differs from western blot in that the assays are conducted in microtiter plates or in vivo rather than on an absorbent membrane.
There are many different types of ELISAs, but they all involve an antibody molecule whose constant region binds an enzyme, leaving the variable region free to bind its specific antigen. Enzyme-substrate reaction allows the antigen to be visualized or...

Immunogold Electron Microscopy

Immunogold Electron Microscopy

Immunoelectron microscopy utilizes immunogold labeling of endogenous proteins with specific antibodies to detect and localize these proteins in cells and tissues. The procedure provides insights into the distribution and quantification of protein under different stimulation conditions offering clues about their functions. Conjugating highly electron-dense gold particles with primary or secondary antibodies allow antigen detection on and within cells, with high resolution and specificity.