一个新的3D打印和小型化的周期性反流色谱系统,用于连续净化单克隆抗体
Carlotta Kortmann1, Taieb Habib1, Christopher Heuer2
1Institute of Technical Chemistry, Leibniz University Hannover, 30167 Hannover, Germany.
Micromachines
|March 28, 2024
概括
使用3D打印制造的一种新型小型化周期逆流色谱 (PCCC) 系统,可显著降低死体积,从而实现高效的蛋白质净化. 这种多功能系统显示出优化生物制药生产流程的前景.
科学领域:
- 生物技术是生物技术.
- 化学工程是化学工程的重要组成部分.
- 分离科学 分离科学
背景情况:
- 连续色谱对于具有成本效益的生物制药生产至关重要,特别是对于单克隆抗体.
- 现有的连续色谱系统通常是庞大的,具有高的死体积,限制了它们的效率.
- 为了改进这些系统,需要优化分离程序.
研究的目的:
- 介绍一个小型的周期反流色谱 (PCCC) 系统.
- 为了证明与传统设置相比,该系统的死体积减少.
- 通过3D打印展示其灵活设计和快速原型设计的潜力.
主要方法:
- 使用3D打印技术制造小型PCCC设备.
- 在单克隆抗体净化 (捕获和抛光步骤) 中展示设备的功能.
- 用亲和和离子交换色谱技术以及用柱子和膜吸附剂测试系统.
主要成果:
- 通过3D打印的PCCC系统显著减少了死亡体积.
- 单克隆抗体净化在捕获和抛光阶段的成功应用.
- 在不同的色谱技术和单元类型中展示了多功能性.
结论:
- 微型PCCC系统为蛋白质净化提供了一个灵活和高效的平台.
- 3D打印使染色学系统的快速原型和设计调整成为可能.
- 这项技术具有优化色谱条件和各种应用的巨大潜力,包括直接捕获产品.
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