双相发酵皮贝的Ganoderma sinense和其多糖和酒精提取物的特征及其生物活性的分析
Xiaoyan Sun1, Qiuqi Lei2, Qinyi Chen1
1College of Life Science and Technology, Wuhan Polytechnic University, Wuhan 430023, China.
Molecules (Basel, Switzerland)
|March 28, 2024
概括
用Ganoderma sinense发酵Trapa bispinosa贝可以增强它们的抗氧化和免疫调节特性. 这一过程转化了多糖和提取物,提高了它们的药用潜力,减少了浪费.
科学领域:
- 生物技术是生物技术.
- 自然产品化学 自然产品化学
- 免疫学 免疫学 免疫学
背景情况:
- 贝 (TBs) 具有宝贵的药用特性,包括抗氧化和免疫调节作用.
- 结核废物经常被丢弃作为农业废物,这代表了资源利用的错失机会.
研究的目的:
- 为了优化Trapa bispinosa贝的固态发酵,使用Ganoderma sinense.
- 评估发酵对结核病提取物和多糖的免疫调节和抗氧化活性的影响.
主要方法:
- 用Ganoderma sinense优化了对结核菌的固态发酵,使用响应表面方法.
- 准备并分析了多糖 (P1,P2) 和酒精提取物 (AE1,AE2) 的活性成分.
- 使用小鼠巨细胞 (RAW 264.7) 评估了免疫刺激和抗氧化活性.
主要成果:
- 最佳的发酵条件 (2天,14°C,77%的湿度) 最大化了水提取产量和DPPH激素清除活动.
- 发酵后的多糖 (P2) 通过NF-κB通路显著增强了免疫细胞分泌物 (NO,IL-2,IL-10,TNF-α).
- 发酵酒精提取物 (AE2) 通过Nrf2/Keap1-ARE通路增强了抗氧化酶 (T-AOC,T-SOD) 和降低了脂质过氧化 (MDA).
结论:
- 通过Ganoderma sinense对TBs的双相发酵有效地改变了多糖体结构和提取物组成.
- 这种发酵过程显著增强了结核病衍生产品的免疫调节和抗氧化活性.
更多相关视频
09:57Separation of Bioactive Small Molecules, Peptides from Natural Sources and Proteins from Microbes by Preparative Isoelectric Focusing IEF Method
Published on: June 14, 2020
9.5K
10:02Isolation and Purification of Fungal β-Glucan as an Immunotherapy Strategy for Glioblastoma
Published on: June 2, 2023
1.9K
相关概念视频
01:25Production of Organic Acids
Lactic acid, an important organic acid extensively applied in food, pharmaceutical, and biodegradable polymer industries, is primarily produced via microbial fermentation. This method is favored over chemical synthesis due to its environmental sustainability and capacity for enantiomerically pure product formation. Among various microbial processes, the fermentation of starch-based substrates stands out due to the abundance and renewability of raw materials like corn and potatoes.Hydrolysis of...
01:15Antifungal Agents
Amphotericin B is a broad-spectrum antifungal agent that exploits structural differences between fungal and mammalian cell membranes. Its amphipathic structure—featuring a hydrophobic polyene-lactone ring and a hydrophilic region containing mycosamine and carboxylic acid groups—enables selective binding to ergosterol, a sterol predominantly found in fungal plasma membranes. This selective interaction underlies the drug’s antifungal activity, although weak binding to cholesterol contributes to...