在人参提取物发酵过程中产生的化合物抗氧化活性的优化 补充了乳酸菌
Shuiqing Lin1,2, Yuxiao Wu2, Qian Huang2
1School of Traditional Chinese Medicine, Guangdong Pharmaceutical University, Guangzhou 510006, China.
Molecules (Basel, Switzerland)
|March 28, 2024
概括
使用乳酸细菌进行优化人参发酵,显著增强了提取物中的抗氧化活性. 发酵的人参通过保护皮肤细胞免受氧化损伤,显示出功能化品的前景.
科学领域:
- 生物技术是生物技术.
- 化品科学 化品科学
- 自然产品化学 自然产品化学
背景情况:
- 人参提取物富含具有药用和化品价值的生物活性化合物.
- 发酵是一种已知的提高化品成分有效性的方法.
- 优化人参发酵可以带来新的功能化品.
研究的目的:
- 为了优化人参茎和叶子提取物的发酵过程,用于功能化品.
- 为了评估发酵的人参提取物的抗氧化潜力.
- 为了验证优化发酵人参在人类皮肤细胞中的有效性.
主要方法:
- 用五种乳酸细菌菌株发酵人参提取物.
- 使用响应表面方法优化发酵参数 (时间,注射剂大小,温度).
- 通过 2,2-diphenyl-1-picrylhydrazyl (DPPH),基 (·OH) 和超氧化 (O2·-) 清除试验来评估抗氧化活性.
- 使用HaCaT和BJ人类皮肤细胞进行体外验证.
主要成果:
- 选择了1.140作为最佳菌株.
- 优化的发酵参数:35.50小时,2.45%的注射剂,28.20°C.
- 抗氧化活性显著增加:DPPH增加了25.00%,OH增加了94.00%,O增加了73.00%.
- 增加了人参化物Rg5的含量,以及特定的人参化物,黄化合物和抗氧化活性之间的相关性.
- 优化的汁促进了细胞增殖,并保护了HaCaT细胞免受氧化损伤.
结论:
- 优化的人参发酵有效地增强了抗氧化特性.
- 发酵的人参提取物显示出预防和修复细胞氧化损伤的潜力.
- 这项研究为在高级功能化品中利用发酵人参提供了基础.
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