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针对Neu5Gc的aptamer的表征,作为来自红肉的内源性致病因子

Yuxi Guo¹, Honglin Ren¹, Han Wang¹

¹State Key Laboratory for Diagnosis and Treatment of Severe Zoonotic Infectious Diseases, Key Laboratory for Zoonosis Research of the Ministry of Education, Institute of Zoonosis, College of Veterinary Medicine, Jilin University, Changchun 130062, China.

Molecules (Basel, Switzerland)

|March 28, 2024

概括

人类不能产生N-glycolylneuraminic酸 (Neu5Gc),这是红肉中发现的化合物. 这项研究开发了一种基于aptamer的敏感测定方法,用于检测肉类中的Neu5Gc,帮助红肉安全研究.

科学领域:

生物化学和分子生物学
食品安全与毒理学
免疫学免疫学免疫学

背景情况:

N-glycolylneuraminic 酸 (Neu5Gc) 是一种非人类合成的酸,它存在于动物来源的食物中,如红肉.
人类对食Neu5Gc的免疫反应可能导致慢性炎症,可能导致心脏病和癌症.
Neu5Gc被认为是一种内源性致病因子,因此其检测对红肉安全至关重要.

研究的目的:

开发和验证一种基于aptamer的新型检测方法,用于红肉中的N-glycolylneuraminic酸 (Neu5Gc).
为了表征一个选定的阿胺体对Neu5Gc.的结合亲和力和特异性.
建立一种灵敏且方便的测定方法,用于量化食品中的Neu5Gc.

主要方法:

使用磁珠SELEX技术准Neu5Gc的阿巴特马的选择.
分析了阿普坦酶同质性,二次结构和结合亲和力 (Ka).
为检测Neu5Gc建立一个酶链接的寡核酸吸附剂试验 (ELOSA).

主要成果:

阿普塔默2B.N2A9表现出高结合亲和力 (Ka = 1.87 × 10^8 L/mol) 和在pH值5.47.4.4范围内对Neu5Gc的特异性.
分子对接证实了阿帕特马2B.N2A9和Neu5Gc.之间的稳定结合相互作用.
开发的ELOSA方法实现了0.71 ng/mL的检测极限,平均恢复率为92.23%.

结论:

该研究成功开发了一种基于aptamer的敏感和特定测定方法,用于检测红肉中的Neu5Gc.
该方法为指导红肉消费和确保食品安全提供了一个实用的工具.
建议对肉类中的Neu5Gc分布进行进一步的研究,以确定其对公共健康的意义.

关键词:

N-葡萄糖神经胺酸 (Neu5Gc) 的使用一个aptamer的应用程序.酶结合的阿普坦酶测定.红肉分析红肉分析

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