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关于在单列并发性奇拉尔-阿基拉尔HPLC分离方法中的奇拉尔静止相的综合性审查

Lajos Attila Papp¹, Zoltán István Szabó^2,3, Gabriel Hancu¹

¹Department of Pharmaceutical Chemistry, Faculty of Pharmacy, "George Emil Palade" University of Medicine, Pharmacy, Science and Technology of Târgu Mures, 540142 Târgu Mureș, Romania.

Molecules (Basel, Switzerland)

|March 28, 2024

概括

现在,基拉静止相 (CSP) 能够同时使用高性能液态染色学 (HPLC) 进行基拉和亚基拉分离. 本文重点介绍了用于各种应用的同时进行酶选择和化学选择分离的方法.

科学领域:

分析化学分析化学
染色体学染色体学是一种染色学.
分离科学分离科学

背景情况:

状静止相 (CSP) 传统上用于通过高性能液态染色学 (HPLC) 进行对抗选择性药物分析.
现代CSP具有显著的化学选择性特性,可实现双重分离机制.
越来越多的趋势集中在开发单列方法,用于同时进行enantioselective和chemoselective分离.

研究的目的:

提供使用CSP的单列同步奇拉-阿奇拉HPLC分离方法的全面审查.
讨论这些先进色谱技术的方法开发,优化和未来的前景.
突出各种CSP类型的化学选择性分离机制.

主要方法:

对采用四种主要CSP类型的报告HPLC方法的审查:循环德克斯特林,糖抗生素,蛋白质和多糖基CSP.
在正常相 (NP),逆相 (RP) 和极性有机 (PO) 染色学模式中分析应用.
对方法开发策略和化学选择性分离机制进行批判性讨论.

主要成果:

在一个单一的列中,CSP促进了同时进行的enantioselective和chemoselective分离.
不同的CSP和色谱模式适用于这些先进的分离技术.
通过了解CSP的双重分离能力,增强了方法开发.

结论:

使用基于CSP的HPLC进行同步的enantio和chemoselective分离是一个有前途的趋势.
尽管了解保留机制存在挑战,但这些方法具有显著的潜力.
药品分析,药物动力学研究和环境监测应用的预期增长.

关键词:

化学选择性化学选择性性静止阶段静止阶段这是enantioselective选择性的.高性能液体色谱学高性能液体色谱学分离机制的分离机制.同时分离的同时分离.

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