在真菌和细菌实验室培养中乙醇和较高酒精的生产以及对法医样本的重要性
Vassiliki A Boumba1, Glykeria Velivasi1, Nikolaos Kourkoumelis2
1Departments of Forensic Medicine & Toxicology, Faculty of Medicine, School of Health Sciences, University of Ioannina, 45500 Ioannina, Greece.
Microorganisms
|March 28, 2024
概括
测试了估计死后血液中微生物乙醇度的数学模型. 细菌模型显示出广泛的适用性,特别是EC模型,有助于确定乙醇.
科学领域:
- 法医毒理学 法医毒理学
- 微生物学 微生物学
- 分析化学 分析化学
背景情况:
- 尸体样本中的乙醇可能源于微生物活动.
- 较高的酒精与微生物乙醇生产相关.
- 存在数学模型来估计微生物乙醇度.
研究的目的:
- 评估细菌和真菌培养中的乙醇和较高的酒精产量.
- 评估现有的数学模型在实验室培养和尸检血液中的适用性.
- 调查模型在例行法医案例工作中的实用性.
主要方法:
- 在各种介质和变质血液中培养细菌 (大肠杆菌,金黄色杆菌,肺炎杆菌,) 和真菌 (C. albicans).
- 使用头空间气体染色学-火焰电离检测 (HS-GC-FID) 量化酒精度.
- 将以前建立的细菌和酵母模型应用于122例尸体解剖病例的培养样本和血液.
主要成果:
- 在测试条件下,某些微生物不能产生特定的酒精 (1-propanol,1-butanol).
- 细菌模型在死后样本中证明了适用性,无论微生物是否存在.
- 在所有测试的细菌和酵母模型中,EC模型表现出最高的适用性.
结论:
- 细菌模型适用于死后样本,而EC模型显示出优异的性能.
- 细菌模型的最佳适用性是在尸检血液中发现的,BAC为0.10-1.0g/L,特别是在暴力/不明原因死亡和腐烂病例中.
- 酵母模型在特定的尸体解剖病例中适用性有限,这表明如果细菌模型适用,微生物乙醇很可能是适用的.
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