在三个代表性包裹病毒中,对某些微生物杀伤性化学物质的敏感性不同
Tanya Kapes1, Charles Quinn1, Andrew Eli Cragun1
1Microbac Laboratories, Inc., Sterling, VA 20164, USA.
Microorganisms
|March 28, 2024
概括
牛病毒性腹病毒 (BVDV) 是封装病毒失活的不良指标,因为它比SARS-CoV-2和疫苗病毒更耐BTC® 835和酸等消毒剂.
科学领域:
- 病毒学 病毒学
- 微生物学 微生物学
- 传染性疾病 传染性疾病
背景情况:
- 脂质包裹病毒是一个多样化的群体,对无活化有不同的敏感性.
- 了解不同的易感性对于有效的消毒策略至关重要.
- 牛病毒性腹病毒 (BVDV),疫苗病毒和SARS-CoV-2是罩病毒无活化研究的相关模型.
研究的目的:
- 为了比较低pH处理和常见的杀菌剂对三种不同的脂质包裹病毒的疗效.
- 为了确定病毒对各种无活化方法的敏感性的潜在差异.
- 为了确定BVDV是否可以在无活化研究中作为其他包裹病毒的可靠替代品.
主要方法:
- 使用BVDV,疫苗病毒和SARS-CoV-2进行了并排液体无活化疗效研究.
- 用5%的动物血清对病毒进行挑战,并暴露在低pH (3.0-3.1),低化 (100ppm),乙醇 (70%),BTC® 835 (100ppm) 和酸 (100ppm) 的环境中.
- 接触时间有所不同:低pH为10分钟和60分钟,微生物杀菌剂为1分钟,按照ASTM E1052标准在室温下进行.
主要成果:
- 低酸和乙醇实现了所有三个病毒的完全无活化.
- 对BTC® 835和酸的敏感性观察到显著差异.
- 敏感性顺序有所变化:BTC® 835 (SARS-CoV-2 > 疫苗病毒 = BVDV),酸 (疫苗病毒 > SARS-CoV-2 > BVDV) 和低pH (疫苗病毒 > SARS-CoV-2 > BVDV).
结论:
- 脂质包裹的病毒对无活化方法不表现出均的敏感性.
- 与疫苗病毒和SARS-CoV-2相比,BVDV对BTC® 835,酸和低pH的耐药性更强.
- 对于评估某些消毒剂对所有包裹病毒的有效性而言,BVDV可能不是一个合适的最坏情况下的替代品.
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