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一种新型调节剂PepR调节了Bacillus thuringiensis中的二胺酶基因pepV的表达

Xin Zhang¹, Hengjie Wang¹, Tinglu Yan¹

¹State Key Laboratory for Biology of Plant Diseases and Insect Pests, Institute of Plant Protection, Chinese Academy of Agricultural Sciences, Beijing 100193, China.

|March 28, 2024

概括

细菌 thuringiensis 调节器 HD73_5014,名为PepR,直接控制二胺酶 pepV 的表达. 这一发现揭示了对细菌基因调节和Bacillus thuringiensis抗生素耐药性机制的新见解.

科学领域:

微生物学微生物学
分子生物学分子生物学
细菌遗传学细菌遗传学

背景情况:

修灵菌产生杀虫剂晶体蛋白质.
一个DeoR家族的转录调节器 (HD73_5014) 之前被证明可以驱动cry1Ac表达.
HD73_5014同类在格拉姆阳性细菌中很常见.

研究的目的:

研究邻近的基因HD73_5014的调节.
确定HD73_5014调节器的功能和目标.
在Bacillus thuringiensis HD73.73中描述pepr基因集群.

主要方法:

用RT-PCR分析转录单元.
促销者-lacZ融合试验用于评估促销者活动.
电泳运动移位测试 (EMSA) 测定直接DNA结合.

主要成果:

rsuA 和 ytgP 基因形成一个操作子,而 pepV 是一个单独的转录单元.
HD73_5014可以调节pepV和rsuA-ytgP促进体.
HD73_5014直接与pepV促进体结合,但不与rsuA-ytgP促进体结合.
HD73_5014被命名为PepR (二胺酶调节剂),因为它可以控制pepV.

结论:

PepR 直接调节了 dipeptidase pepV. 的表达.
佩普R和佩普V在Bacillus thuringiensis中对万科胺素敏感性起作用.
这些发现扩大了对Bacillus thuringiensis转录调节的理解.

关键词:

细菌 thuringiensis 这种疾病.这种基因是 dipeptidase 基因 pepV pepV.耐药的万科米辛耐药性耐药性的万科米辛转录调节器PepR

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