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单次MVA-SARS-2-ST/N疫苗接种迅速保护K18-hACE2小鼠免受致命的SARS-CoV-2挑战感染

Sabrina Clever¹, Leonard Limpinsel², Christian Meyer Zu Natrup¹

¹Institute of Virology, University of Veterinary Medicine Hannover, Buenteweg 17, 30559 Hanover, Germany.

|March 28, 2024

概括

一种新型的MVA疫苗共同表达SARS-CoV-2尖峰 (ST) 和核蛋白 (N) 在小鼠中表现出强大的抗致命的COVID-19保护. 这种多价值疫苗战略显示出对新出现的传染病快速保护的承诺.

科学领域:

疫苗学疫苗学疫苗学
病毒学病毒学
免疫学免疫学免疫学

背景情况:

由于SARS-CoV-2的出现,需要快速开发疫苗以应急准备.
现有的疫苗策略需要加强,以提高免疫性和疗效.

研究的目的:

开发和评估一种复合修饰疫苗安卡拉 (MVA) 疫苗,该疫苗既能表达SARS-CoV-2前输液稳定尖峰 (ST),也能表达核蛋白 (N).
评估疫苗在小鼠模型中诱导致命SARS-CoV-2挑战的保护性免疫的能力.

主要方法:

开发一种重组MVA疫苗 (MVA-SARS-2-ST/N),该疫苗可以同时表达SARS-CoV-2的ST和N蛋白.
接种K18-hACE2小鼠的疫苗,随后进行致命的SARS-CoV-2挑战.
评估中和抗体 (nAB) 和和肺中的SARS-CoV-2特异性T细胞反应.

主要成果:

一次MVA-SARS-2-ST/N疫苗接种为28天后对致命的SARS-CoV-2挑战提供了强大的保护.
接种疫苗诱导了显著的SARS-CoV-2中和抗体和T细胞反应,特别是在肺部.
紧急疫苗接种 (挑战前2天) 延迟了疾病的发病,并促进了nAB和T细胞的反应.

结论:

一种共同表达SARS-CoV-2S和N蛋白质的多价值MVA疫苗为快速保护提供了一个有前途的战略.
这种方法有助于制定有效的疫苗接种策略来应对新出现的病毒威胁.

关键词:

在K18-hACE2小鼠中.这就是SARS-CoV-2病毒.是一种多价值疫苗.麻疹病毒 (poxvirus) 是一种病毒.

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