布林西多福维尔通过破坏病毒复制来有效抑制伪狂犬病病毒的扩散
Huihui Guo1,2, Qingyun Liu1,2, Dan Yang1,2
1National Key Laboratory of Agricultural Microbiology, College of Veterinary Medicine, Huazhong Agricultural University, Wuhan 430070, China.
Viruses
|March 28, 2024
概括
伪狂热病毒 (PRV) 在动物和人类中引起严重疾病. 这项研究对FDA批准的药物进行了选,确定了布林西多福维尔作为一种有希望的抗病毒药物,可以有效地对抗PRV感染.
科学领域:
- 病毒学 病毒学
- 传染性疾病 传染性疾病
- 药物发现 药物发现 药物发现
背景情况:
- 伪狂热病毒 (PRV) 造成重大经济损失,对动物和人类健康构成威胁.
- 现有的抗疹病毒药物对PRV的疗效有限.
- 迫切需要有效的PRV治疗方法.
研究的目的:
- 开发一种高通量查方法,用于识别针对PRV.的新型抗病毒药物.
- 评估FDA批准的小分子药物对PRV感染的疗效.
- 为了确定PRV治疗的潜在治疗候选者.
主要方法:
- 使用光标记的PRV菌株开发了一种高通量选方法.
- 2104 FDA批准的小分子药物被选为PRV抑制.
- 有前途的候选药物进一步通过体外试验 (细胞病变效应,病毒标位,基因表达) 和体内小鼠模型进行了验证.
主要成果:
- 104种药物显示了超过75%的PRV光强度抑制.
- 10种药物在体外显著抑制了PRV的扩散,具有高选择性指数 (26.3-3937.2).
- 布林西多福维尔在感染致命PRV菌株的小鼠中显示出100%的保护,显著减少了症状和病理.
结论:
- 布林西多福维尔在体外和体内有效抑制PRV复制.
- 布林西多福维尔在病毒复制阶段干扰PRV的扩散.
- 布林西多福维尔 (Brincidofovir) 是一种有希望的候选药物,用于临床治疗PRV感染.
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