相关实验视频

Updated: Jun 26, 2026

Introducing Point Mutations into Human Pluripotent Stem Cells Using Seamless Genome Editing
09:03

Introducing Point Mutations into Human Pluripotent Stem Cells Using Seamless Genome Editing

Published on: May 10, 2020

4.1K

转基因人类前皮纤维细胞系 (YhFF#8) 稳定表达Cas9基因:实验室资源报告

Farzad Soheilipour1,2, Sohrab Boozarpour2, Shiva Aghaei1

  • 1Stem Cell Biology Research Center, Yazd Reproductive Sciences Institute, Shahid Sadoughi University of Medical Sciences, Yazd, Iran.

International journal of reproductive biomedicine
|March 28, 2024
PubMed
概括

研究人员开发了一种稳定的人类纤维细胞细胞系,表达Cas9 (CRISPR相关蛋白9) 以有效编辑基因. 这种新的细胞系有助于疾病建模和治疗开发,简化了遗传研究.

关键词:
这就是CRISPR-Cas9.9.细胞系细胞系的细胞系遗传转导是一种基因转导.纤维细胞细胞.

更多相关视频

Development of Knock-Out Muscle Cell Lines using Lentivirus-Mediated CRISPR/Cas9 Gene Editing10:12

Development of Knock-Out Muscle Cell Lines using Lentivirus-Mediated CRISPR/Cas9 Gene Editing

Published on: June 16, 2022

4.2K
CRISPR-Cas9 Mediated Gene Deletion in Human Pluripotent Stem Cells Cultured Under Feeder-Free Conditions04:21

CRISPR-Cas9 Mediated Gene Deletion in Human Pluripotent Stem Cells Cultured Under Feeder-Free Conditions

Published on: November 1, 2024

657

相关概念视频

CRISPR/Cas9 Genome Editing01:28

CRISPR/Cas9 Genome Editing

The CRISPR-Cas system serves as a bacterial defense mechanism against invading genetic elements such as viruses and plasmids, forming the foundation for its adaptation as a powerful genome-editing tool. Originally discovered in prokaryotes, this system has been repurposed to revolutionize genetic engineering across a wide range of organisms, including plants, animals, and humans. The core component, Cas9, is an endonuclease derived from Streptococcus pyogenes, capable of introducing...