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Updated: Jun 29, 2025

Isolation of Sertoli Cells and Peritubular Cells from Rat Testes

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Published on: February 8, 2016

塞尔托利细胞有条件的介质可以改善血液丸屏障功能和精子生成在由阴囊热过高诱导的亚子精子症小鼠:一个实验研究实验研究

Fakhroddin Aghajanpour^1,2, Reza Soltani^1,2, Azar Afshar^1,2

¹Department of Biology and Anatomical Sciences, School of Medicine, Shahid Beheshti University of Medical Sciences, Tehran, Iran.

International journal of reproductive biomedicine

|March 28, 2024

概括

塞尔托利细胞调节介质 (SCCM) 改善了丸热应激小鼠的精子生产和质量. 此外,SCCM还增强了血液丸屏障功能和生殖细胞发育.

科学领域:

生殖生物学生殖生物学
安德罗学与人类学
细胞生物学细胞生物学

背景情况:

丸体温升高会损害生殖管上皮质,并破坏精子生产.
阴囊温度升高是男性不孕症的已知危险因素.

研究的目的:

研究塞尔托利细胞条件介质 (SCCM) 的治疗潜力,以减轻阴囊热过热引起的丸损伤.
评估SCCM对血液丸屏障 (BTB) 相关基因和小鼠模型中的精子生成的影响.

主要方法:

成年NMRI小鼠接受了阴囊热量升高 (10天每天20分钟温度为43°C).
在5周的时间里,小鼠每隔一天接受Dulbecco的修饰介质 (DMEM) 或SCCM (10微升).
分析了精子参数,丸组织学,立体学,氧化应激标志物 (ROS,谷氨) 和BTB基因 (Ocln,Gja1,Cdh2,Itgb1) 的表达.

主要成果:

与只有高温症的组相比,SCCM治疗显著增加了精子数量和运动性,同时减少了DNA碎片化.
组织学和立体学评估显示,SCCM治疗小鼠的丸体积和生殖细胞数量增加.
施用SCCM提高了关键的血液-丸屏障基因 (Ocln,Gja1,Cdh2,Itgb1) 的表达,并增加了谷氨水平.

结论:

在小鼠中,SCCM证明了对囊热过热引起的丸损伤的保护作用.
在SCCM内部的生长因子通过膜信号促进胚胎细胞的增殖和分化.
在热应激条件下,SCCM有效地提高了与血液丸屏障相关的基因的调节,恢复了屏障完整性.

关键词:

文化媒体文化热量过高的情况.塞尔托利细胞是Sertoli细胞.精子发生.精子生成.测试 (Testis) 是一个测试.

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